지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 87 - 91 (5page)
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Purpose: The aim of this study was to evaluate the diagnostic value of a peptide nucleic acid (PNA)-mediated PCR clamping method for the detection of BRAFV600E mutations in fine needle aspiration cytology (FNAC).
Methods: One hundred sixty four patients underwent FNAC to evaluate BRAFV600E mutations between April 2011 and November 2011. Among them, forty-two patients were diagnosed with papillary thyroid carcinoma in a permanent pathologic specimen. A PNA-mediated PCR clamping method and a Dual-Priming Oligonucleotide (DPO)-based Real-time PCR method were used to detect the BRAFV600E mutation. We compared the result of mutation between the two methods.
Results: A BRAF mutation was found in 31 samples created by the PNA-mediated PCR clamping method, and in 28 samples in the DPO-based Real-time PCR method. The rate of BRAF mutation was 73.8% in association with the PNA-mediated PCR clamping method, and 66.7% in association with the DPO-based Real-time PCR method. There was no statistical differences between the two methods (P＞0.05).
Conclusion: The PNA-mediated PCR clamping method may be an alternative to the DPO-based Real-Time PCR method for detection of BRAF mutations in thyroid nodules.
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참고문헌 (16)
참고문헌 신청
Feinstein E / 1992 / Expression of the normal p53 gene induces differentiation of K562 cells / Oncogene 7 : 1853 ~ 1857
Flaherty KT / 2010 / Mutation-driven drug development in melanoma / Curr Opin Oncol 22 : 178 ~ 183
Frasca F / 2008 / BRAF(V600E) mutation and the biology of papillary thyroid cancer / Endocr Relat Cancer 15 : 191 ~ 205
Fusco A / 1987 / A new oncogene in human thyroid papillary carcinomas and their lymph-nodal metastases / Nature 328 : 170 ~ 172
Hamacher C / 1995 / Expression of functional stimulatory guanine nucleotide binding protein in nonfunctioning thyroid adenomas is not correlated to adenylate cyclase activity
Flaherty KT / 2010 / Mutation-driven drug development in melanoma / Curr Opin Oncol 22 : 178 ~ 183
Frasca F / 2008 / BRAF(V600E) mutation and the biology of papillary thyroid cancer / Endocr Relat Cancer 15 : 191 ~ 205
Fusco A / 1987 / A new oncogene in human thyroid papillary carcinomas and their lymph-nodal metastases / Nature 328 : 170 ~ 172
Hamacher C / 1995 / Expression of functional stimulatory guanine nucleotide binding protein in nonfunctioning thyroid adenomas is not correlated to adenylate cyclase activity
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