지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Background: Estrogens exert preferable effects on bone metabolism through two es-trogen receptors (ERs), ER1 and ER2, which activate the transcription of a set of genes as ligand-dependent transcription factors. Thus, growth factors and hormones which mod-ulate ER expression in the bone, if any, may possibly modulate the effect of estrogens on bone metabolism. However, research as to which of these molecules regulate the ex-pression of ERs in osteoblasts has not been well documented. Methods: A reporter as-say system developed in this study was used to explore molecules that modulate ER1expression in MC3T3-E1 osteoblastic cells. Gene expression was analyzed by reverse transcription-polymerase chain reaction. Results: A pilot study using the reporter sys-tem revealed that bone morphogenetic protein (BMP)-2 negatively regulated ER1, but not ER2, expression in MC3T3-E1 cells. Consistently, estradiol-induced reporter activity via an estrogen responsive element was strongly suppressed in MC3T3-E1 cells pretreat-ed with BMP-2. Conclusions: BMP-2 desensitizes osteoblastic cells to estrogen through downregulation of ER1 expression.
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참고문헌 (24)
참고문헌 신청
Bland R / 2000 / Steroid hormone receptor expression and action in bone / Clin Sci (Lond) 98 : 217 ~ 240
Emmen JM / 2003 / Estrogen receptor knockout mice: phenotypes in the female reproductive tract / Gynecol Endocrinol 17 : 169 ~ 176
Hawse JR / 2008 / Estrogen-TGFbeta cross-talk in bone and other cell types: role of TIEG, Runx2, and other transcription factors / J Cell Biochem 103 : 383 ~ 392
Heldring N / 2007 / Estrogen receptors: how do they signal and what are their targets / Physiol Rev 87 : 905 ~ 931
Hewitt SC / 2003 / Oestrogen receptor knockout mice: roles for oestrogen receptors alpha and beta in reproductive tissues / Reproduction 125 : 143 ~ 149
Emmen JM / 2003 / Estrogen receptor knockout mice: phenotypes in the female reproductive tract / Gynecol Endocrinol 17 : 169 ~ 176
Hawse JR / 2008 / Estrogen-TGFbeta cross-talk in bone and other cell types: role of TIEG, Runx2, and other transcription factors / J Cell Biochem 103 : 383 ~ 392
Heldring N / 2007 / Estrogen receptors: how do they signal and what are their targets / Physiol Rev 87 : 905 ~ 931
Hewitt SC / 2003 / Oestrogen receptor knockout mice: roles for oestrogen receptors alpha and beta in reproductive tissues / Reproduction 125 : 143 ~ 149
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