지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 586 - 593 (8page)
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초록· 키워드
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Reactive oxygen species (ROS) contribute to the development of a number of neuronal diseases including ischemia. DJ-1, also known to PARK7, plays an important role in transcriptional regulation, acting as molecular chaperone and antioxidant. In the present study, we investigated whether DJ-1 protein shows a protective effect against oxidative stress-induced neuronal cell death in vitro and in ischemic animal models in vivo. To explore DJ-1 protein’s potential role in protecting against ischemic cell death, we constructed cell permeable Tat-DJ-1 fusion proteins. Tat-DJ-1 protein efficiently transduced into neuronal cells in a doseand time-dependent manner. Transduced Tat-DJ-1 protein increased cell survival against hydrogen peroxide (H2O2) toxicity and also reduced intracellular ROS. In addition, Tat-DJ-1 protein inhibited DNA fragmentation induced by H2O2. Furthermore, in animal models, immunohistochemical analysis revealed that Tat-DJ-1 protein prevented neuronal cell death induced by transient forebrain ischemia in the CA1 region of the hippocampus. These results demonstrate that transduced Tat-DJ-1 protein protects against cell death in vitro and in vivo, suggesting that the transduction of Tat-DJ-1 may be useful as a therapeutic agent for ischemic injuries related to oxidative stress.
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참고문헌 (26)
참고문헌 신청
[학술지(정기간행물)]
Alexandrova M / 2004 / Dynamics of free radical processes in acute ischemic stroke: influence on neurological status and outcome / J Clin Neurosci 11 : 501 ~ 506
Aleyasin H / 2007 / The Parkinson’s disease gene DJ-1 is also a key regulator of stroke-induced damage / Proc Natl Acad Sci USA 104 : 18748 ~ 18753
An JJ / 2008 / Transduced human PEP-1-heat shock protein 27 efficiently protects against brain ischemic insult / FEBS J 275 : 1296 ~ 1308
Andersen JK / 2004 / Oxidative stress in neurodegeneration: cause or consequence? / Nat Med 10 : S18 ~ S25
Alexandrova M / 2004 / Dynamics of free radical processes in acute ischemic stroke: influence on neurological status and outcome / J Clin Neurosci 11 : 501 ~ 506
Aleyasin H / 2007 / The Parkinson’s disease gene DJ-1 is also a key regulator of stroke-induced damage / Proc Natl Acad Sci USA 104 : 18748 ~ 18753
An JJ / 2008 / Transduced human PEP-1-heat shock protein 27 efficiently protects against brain ischemic insult / FEBS J 275 : 1296 ~ 1308
Andersen JK / 2004 / Oxidative stress in neurodegeneration: cause or consequence? / Nat Med 10 : S18 ~ S25
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