지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 1 - 10 (10page)
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초록· 키워드
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The microRNA (miRNA) regulation mechanisms associated with atherosclerosis are largely undocumented. Specific selection and efficient validation of miRNA regulation pathways involved in atherosclerosis development may be better assessed by contemporary microarray platforms applying cross-verification methodology. A screening platform was established using both miRNA and genomic microarrays. Microarray analysis was then simultaneously performed on pooled atherosclerotic aortic tissues from 10 Apolipoprotein E (apoE) knockout mice (apoE / ) and 10 healthy C57BL/6 (B6) mice. Differentiated miRNAs were screened and cross-verified against an mRNA screen database to explore integrative mRNA–miRNA regulation. Gene set enrichment analysis was conducted to describe the potential pathways regulated by these mRNA–miRNA interactions.
High-throughput data analysis of miRNA and genomic microarrays of knockout and healthy control mice revealed 75differentially expressed miRNAs in apoE / mice at a threshold value of 2. The six miRNAs with the greatest differentiation expression were confirmed by real-time quantitative reverse-transcription PCR (qRT–PCR) in atherosclerotic tissues.
Significantly enriched pathways, such as the type 2 diabetes mellitus pathway, were observed by a gene-set enrichment analysis. The enriched molecular pathways were confirmed through qRT–PCR evaluation by observing the presence of suppressor of cytokine signaling 3 (SOCS3) and SOCS3-related miRNAs, miR-30a, miR-30e and miR-19b. Cross-verified highthroughput microarrays are optimally accurate and effective screening methods for miRNA regulation profiles associated with atherosclerosis. The identified SOCS3 pathway is a potentially valuable target for future development of targeted miRNA therapies to control atherosclerosis development and progression.
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참고문헌 (24)
참고문헌 신청
Bonenfant M / 2000 / Localization of type 1 17beta-hydroxysteroid dehydrogenase mRNA and protein in syncytiotrophoblasts and invasive cytotrophoblasts in the human term villi
Chen T / 2009 / MicroRNA-125a-5p partly regulates the inflammatory response, lipid uptake, and ORP9 expression in oxLDL-stimulated monocyte/macrophages / Cardiovasc Res 83 :
Cheng Y / 2007 / MicroRNAs are aberrantly expressed in hypertrophic heart: do they play a role in cardiac hypertrophy? / Am J Pathol 170 : 1831 ~ 1840
Eisenberg I / 2007 / Distinctive patterns of microRNA expression in primary muscular disorders / Proc Natl Acad Sci USA 104 : 17016 ~ 17021
Fang Y / 2010 / MicroRNA-10a regulation of proinflammatory phenotype in athero-susceptible endothelium in vivo and in vitro / Proc Natl Acad Sci USA 107 : 13450 ~ 13455
Chen T / 2009 / MicroRNA-125a-5p partly regulates the inflammatory response, lipid uptake, and ORP9 expression in oxLDL-stimulated monocyte/macrophages / Cardiovasc Res 83 :
Cheng Y / 2007 / MicroRNAs are aberrantly expressed in hypertrophic heart: do they play a role in cardiac hypertrophy? / Am J Pathol 170 : 1831 ~ 1840
Eisenberg I / 2007 / Distinctive patterns of microRNA expression in primary muscular disorders / Proc Natl Acad Sci USA 104 : 17016 ~ 17021
Fang Y / 2010 / MicroRNA-10a regulation of proinflammatory phenotype in athero-susceptible endothelium in vivo and in vitro / Proc Natl Acad Sci USA 107 : 13450 ~ 13455
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