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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2004.1
- 수록면
- 145 - 156 (12page)
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In this study, Leishmania RNA virus 1-4 (LRV1-4)
particles purified from host Leishmania guyanensis
promastigotes were examined for capsid endoribonuclease.
Temperature optimum for the endoribonulease
activity was found to be at 37oC to
42oC and the activity was specifically inhibited by
the aminoglycoside antibiotics, neomycin, kanamycin,
and hygromycin and by 100 mM levels of NaCl
or KCl. To determ ine the catalytic domain of the
capsid endoribonuclease activity, three point-mutation
at cysteine residues at C47S (P1), C128/
133S (P2), and C194R (P3) were prepared and each
gene was constructed into baculoviruses and
expressed in Sf9 insect cells. LRV1-4 capsid Nterminus
(N2 and N3) and C-terminus (C1 and C2)
deletion mutants (Cadd et al., 1994) were also
examined by in vitro RNA cleavage assay. The
results showed that the capsid mutants; C1, C2,
N3, P1, and P2 were capable of forming proper
virus-like particles (VLPs) and they all possessed
the specific endoribonuclease activity. However,
two assembly-defective capsid mutants, N2 (Nterminus
24-amino acids deletion) and P3 mutants,
did not retain the specific endoribonuclease activity.
Taken together, the results suggest that at
least 24 amino acids from the N-terminal region
and C194 residue in LRV1-4 capsid protein are
functionally important for LRV1-4 viral assembly
and the capsid endoribonuclease activity may be
dependent upon the properly assembled LRV1-4
virus particles.
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등록된 정보가 없습니다.
참고문헌 (30)
참고문헌 신청
/ / Anticodon loop of tRNAPhe : 6365 ~ 71
/ / Dual interference ofhygromycin B with ribosomal translocation and with aminoacyl-tRNA recognition : 21 ~ 7
/ / hygromycin B is an atypical bactericidal compound thatexerts effects on cells of Escherichia coli characteristic forbacteriostatic aminocyclitols ami (glycoside and amicy
/ 1993 / RNA processing and degradation by RNase III InControl of messenger RNA stability : 71 ~ 116
/ 1993 / mRNA degradation in prokaryotic cells In Control of messenger RNA stability : 3 ~ 12
/ / Dual interference ofhygromycin B with ribosomal translocation and with aminoacyl-tRNA recognition : 21 ~ 7
/ / hygromycin B is an atypical bactericidal compound thatexerts effects on cells of Escherichia coli characteristic forbacteriostatic aminocyclitols ami (glycoside and amicy
/ 1993 / RNA processing and degradation by RNase III InControl of messenger RNA stability : 71 ~ 116
/ 1993 / mRNA degradation in prokaryotic cells In Control of messenger RNA stability : 3 ~ 12
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