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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
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- 1 - 9 (9page)
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Human mesenchymal stem cells (MSCs) have emerged as attractive cellular vehicles to deliver therapeutic genes for ex-vivo therapy of diverse diseases; this is, in part, because they have the capability to migrate into tumor or lesion sites. Previously,we showed that MSCs could be utilized to deliver a bacterial cytosine deaminase (CD) suicide gene to brain tumors. Here we assessed whether transduction with a retroviral vector encoding CD gene altered the stem cell property of MSCs. MSCs were transduced at passage 1 and cultivated up to passage 11. We found that proliferation and differentiation potentials,chromosomal stability and surface antigenicity of MSCs were not altered by retroviral transduction. The results indicate that retroviral vectors can be safely utilized for delivery of suicide genes to MSCs for ex-vivo therapy. We also found that a single retroviral transduction was sufficient for sustainable expression up to passage 10. The persistent expression of the transduced gene indicates that transduced MSCs provide a tractable and manageable approach for potential use in allogeneic transplantation.
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참고문헌 (22)
참고문헌 신청
Aizman I / 2009 / Extracellular matrix produced by bone marrow stromal cells and by their derivative, SB623 cells, supports neural cell growth / J Neurosci Res 87 : 3198 ~ 3
Amado LC / 2005 / Cardiac repair with intramyocardial injection of allogeneic mesenchymal stem cells after myocardial infarction / Proc Natl Acad Sci USA 102 : 11474 ~ 11479
Armstrong RJ / 2000 / Survival, neuronal differentiation, and fiber outgrowth of propagated human neural precursor grafts in an animal model of Huntington’s disease / Cell T
Bai L / 2012 / Hepatocyte growth factor mediates mesenchymal stem cell-induced recovery in multiple sclerosis models / Nat Neurosci 15 : 862 ~ 870
Bouchez G / 2008 / Partial recovery of dopaminergic pathway after graft of adult mesenchymal stem cells in a rat model of Parkinson’s disease / Neurochem Int 52 : 1332 ~ 134
Amado LC / 2005 / Cardiac repair with intramyocardial injection of allogeneic mesenchymal stem cells after myocardial infarction / Proc Natl Acad Sci USA 102 : 11474 ~ 11479
Armstrong RJ / 2000 / Survival, neuronal differentiation, and fiber outgrowth of propagated human neural precursor grafts in an animal model of Huntington’s disease / Cell T
Bai L / 2012 / Hepatocyte growth factor mediates mesenchymal stem cell-induced recovery in multiple sclerosis models / Nat Neurosci 15 : 862 ~ 870
Bouchez G / 2008 / Partial recovery of dopaminergic pathway after graft of adult mesenchymal stem cells in a rat model of Parkinson’s disease / Neurochem Int 52 : 1332 ~ 134
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