지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 1 - 7 (7page)
이용수
초록· 키워드
오류제보하기
New colchicine analogs have been synthesized with the aim of developing stronger potential anticancer activities. Among the analogs, CT20126 has been previously reported to show immunosuppressive activities. Here, we report that CT20126 also shows potential anticancer effects via an unusual mechanism: the modulation of microtubule integrity and cell cycle arrest at the G2/M phase before apoptosis. When we treated COS-7 cells with CT20126 (5 lM), the normal thread-like microtubules were disrupted into tubulin dimers within 10min and thereafter repolymerized into short, thick filaments. In contrast, cells treated with the same concentration of colchicine exhibited microtubule depolymerization after 20min and never underwent repolymerization.
Furthermore, optical density (OD) analysis (350nm) with purified tubulin showed that CT20126 had a higher repolymerizing activity than that of Taxol, a potent microtubule-polymerizing agent. These results suggest that the effects of CT20126 on microtubule integrity differ from those of colchicine: the analog first destabilizes microtubules and then stabilizes the disrupted tubulins into short, thick polymers. Furthermore, CT20126 induced a greater level of apoptotic activity in Jurkat T cells than colchicine (assessed by G2/M arrest, caspase-3 activation and cell sorting). At 20nM, CT20126 induced 47% apoptosis among Jurkat T cells, whereas colchicine induced only 33% apoptosis. Our results suggest that the colchicine analog CT20126 can potently induce apoptosis by disrupting microtubule integrity in a manner that differs from that of colchicine or Taxol.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
A
Abdullah NM / 2009 / Selective targeting of tumorigenic cancer cell lines by microtubule inhibitors / PLoS One 4 : e4470 ~
Andreu JM / 1998 / Role of the colchicine ring A and its methoxy groups in the binding to tubulin and microtubule inhibition / Biochemistry 37 : 8356 ~ 8368
Bae SS / 2000 / Proteolytic cleavage of phospholipase C-gamma1 during apoptosis in Molt-4 cells / FASEB J 14 : 1083 ~ 1092
Ben-Zvi I / 2012 / Risk factors for amyloidosis and impact of kidney transplantation on the course of familial Mediterranean fever / Isr Med Assoc J 14 : 221 ~ 224
Abdullah NM / 2009 / Selective targeting of tumorigenic cancer cell lines by microtubule inhibitors / PLoS One 4 : e4470 ~
Andreu JM / 1998 / Role of the colchicine ring A and its methoxy groups in the binding to tubulin and microtubule inhibition / Biochemistry 37 : 8356 ~ 8368
Bae SS / 2000 / Proteolytic cleavage of phospholipase C-gamma1 during apoptosis in Molt-4 cells / FASEB J 14 : 1083 ~ 1092
Ben-Zvi I / 2012 / Risk factors for amyloidosis and impact of kidney transplantation on the course of familial Mediterranean fever / Isr Med Assoc J 14 : 221 ~ 224
이 논문의 저자 정보
최근 본 자료
댓글(0)
0