지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
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The N-terminal fragment of prohormone brain natriuretic peptide (NT-proBNP) is a commonly used biomarker for the diagnosis of congestive heart failure, although its biological function is not well known. NT-proBNP exhibits heavy O-linked glycosylation, and it is quite difficult to develop an antibody that exhibits glycosylation-independent binding. We developed an antibody that binds to the recombinant NT-proBNP protein and its deglycosylated form with similar affinities in an enzyme immunoassay. The epitope was defined as Gly63–Lys68 based on mimetic peptide screening, site-directed mutagenesis and a competition assay with a peptide mimotope. The nearest O-glycosylation residues are Thr58 and Thr71; therefore, four amino acid residues intervene between the epitope and those residues in both directions. In conclusion, we report that an antibody reactive to Gly63–Lys68 of NT-proBNP exhibits O-glycosylation-independent binding.
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참고문헌 (21)
참고문헌 신청
Barbas CF III / 2001 / Phage Display: A Laboratory Manual / Cold Spring Harbor Laboratory Press
Bayes-Genis A / 2004 / N-terminal probrain natriuretic peptide(NT-proBNP)in the emergency diagnosis and in-hospital monitoring of patients with dyspnoea and ventricular dysfu
Biomedica Medizinprodukte Gmbh / 2010 / Method of determining NT-proBNP. US patent 20100136590A1
Boussif O / 1995 / A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo : polyethylenimine / Proc Natl Acad Sci USA 92 : 7297 ~ 7301
Chung J / 2002 / Identification of antigenic peptide recognized by the anti-JL1 leukemia-specific monoclonal antibody from combinatorial peptide phage display libraries / J
Bayes-Genis A / 2004 / N-terminal probrain natriuretic peptide(NT-proBNP)in the emergency diagnosis and in-hospital monitoring of patients with dyspnoea and ventricular dysfu
Biomedica Medizinprodukte Gmbh / 2010 / Method of determining NT-proBNP. US patent 20100136590A1
Boussif O / 1995 / A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo : polyethylenimine / Proc Natl Acad Sci USA 92 : 7297 ~ 7301
Chung J / 2002 / Identification of antigenic peptide recognized by the anti-JL1 leukemia-specific monoclonal antibody from combinatorial peptide phage display libraries / J
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