지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 1 - 10 (10page)
이용수
초록· 키워드
오류제보하기
In women with preeclampsia (PE), endothelial cell (EC) dysfunction can lead to altered secretion of paracrine factors that induce peripheral vasoconstriction and proteinuria. This study examined the hypothesis that PE sera may directly or indirectly, through human umbilical vein ECs (HUVECs), stimulate phospholipase C-γ1-1,4,5-trisphosphate (PLC-γ1-IP3) signaling, thereby increasing protein kinase C-α (PKC-α) activity, collagen I expression and intracellular Ca2+ concentrations ([Ca2+]i) in human umbilical artery smooth muscle cells (HUASMCs). HUASMCs and HUVECs were cocultured with normal or PE sera before PLC-γ1 silencing. Increased PLC-γ1 and IP3 receptor (IP3R) phosphorylation was observed in cocultured HUASMCs stimulatedwith PE sera (Po0.05). In addition, PE serum significantly increased HUASMC viability and reduced their apoptosis (Po0.05); these effects were abrogated with PLC-γ1 silencing. Compared with normal sera, PE sera increased [Ca2+]i in cocultured HUASMCs (Po0.05), which was inhibited by PLC-γ1 and IP3R silencing. Finally, PE sera-induced PKC-α activity and collagen I expression was inhibited by PLC-γ1 small interfering RNA (siRNA) (Po0.05). These results suggest that vasoactive substances in the PE serum may induce deposition in the extracellular matrix through the activation of PLC-γ1, which may in turn result in thickening and hardening of the placental vascular wall, placental blood supply shortage, fetal hypoxia–ischemia and intrauterine growth retardation or intrauterine fetal death. PE sera increased [Ca2+]i and induced PKC-α activation and collagen I expression in cocultured HUASMCs via the PLC-γ1 pathway.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
[학술지(정
Chandra A / 2006 / VEGF inhibits PDGF-stimulated calcium signaling independent of phospholipase C and protein kinase C / J Surg Res 131 : 302 ~ 309
Fan C / 2009 / Hypoxia-induced mitogenic factor/FIZZ1 induces intracellular calcium release through the PLC-IP(3) pathway / Am J Physiol Lung Cell Mol Physiol 297 : L263 ~ L2
Goel SA / 2013 / Preferential secretion of collagen type 3 versus type 1 from adventitial fibroblasts stimulated by TGF-β/Smad3-treated medial smooth muscle cells / Cell Si
Higuchi S / 2007 / Angiotensin II signal transduction through the AT1 receptor : novel insights into mechanisms and pathophysiology / Clin Sci(Lond) 112 : 417 ~ 428
Chandra A / 2006 / VEGF inhibits PDGF-stimulated calcium signaling independent of phospholipase C and protein kinase C / J Surg Res 131 : 302 ~ 309
Fan C / 2009 / Hypoxia-induced mitogenic factor/FIZZ1 induces intracellular calcium release through the PLC-IP(3) pathway / Am J Physiol Lung Cell Mol Physiol 297 : L263 ~ L2
Goel SA / 2013 / Preferential secretion of collagen type 3 versus type 1 from adventitial fibroblasts stimulated by TGF-β/Smad3-treated medial smooth muscle cells / Cell Si
Higuchi S / 2007 / Angiotensin II signal transduction through the AT1 receptor : novel insights into mechanisms and pathophysiology / Clin Sci(Lond) 112 : 417 ~ 428
이 논문의 저자 정보
최근 본 자료
댓글(0)
0