지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 268 - 280 (13page)
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초록· 키워드
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DNA methylation may regulate gene expression by restricting the access of transcription factors. We have previously demonstrated that GATA-1 regulates the transcription of the CCR3 gene by dynamically interacting with both positively and negatively acting GATA elements of high affinity binding in the proximal promoter region including exon 1. Exon 1 has three CpG sites, two of which are positioned at the negatively acting GATA elements. We hypothesized that the methylation of these two CpGs sites might preclude GATA-1binding to the negatively acting GATA elements and,as a result, increase the availability of GATA-1 to the positively acting GATA element, thereby contributing to an increase in GATA-1-mediated transcription of the gene. To this end, we determined the methylation of the three CpG sites by bisulfate pyrosequencing in peripheral blood eosinophils, cord blood (CB)-derived eosinophils,PBMCs, and cell lines that vary in CCR3 mRNA expression. Our results demonstrated that methylation of CpG sites at the negatively acting GATA elements severely reduced GATA-1 binding and augmented transcription activity in vitro. In agreement,methylation of these CpG sites positively correlated with CCR3 mRNA expression in the primary cells and cell lines examined. Interestingly, methylation patterns of these three CpG sites in CB-derived eosinophils mostly resembled those in peripheral blood eosinophils. These results suggest that methylation of CpG sites at the GATA elements in the regulatory regions fine-tunes CCR3 transcription.
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참고문헌 (24)
참고문헌 신청
Aoki M / 2008 / Kidney-specific expression of human organic cation transporter 2 (OCT2/SLC22A2) is regulated by DNA methylation / Am J Physiol Renal Physiol 295 : F165 ~ F170
Beck LA / 2006 / Functional analysis of the chemokine receptor CCR3 on airway epithelial cells / J Immunol 177 : 3344 ~ 3354
Bell AC / 2000 / Methylation of a CTCF-dependent boundary controls imprinted expression of the Igf2 gene / Nature 405 : 482 ~ 485
Broske AM / 2009 / DNA methylation protects hematopoietic stem cell multipotency from myeloerythroid restriction / Nat Genet 41 : 1207 ~ 1215
Bystrom J / 2004 / Gene microarray analysis reveals interleukin-5-dependent transcriptional targets in mouse bone marrow / Blood 103 : 868 ~ 877
Beck LA / 2006 / Functional analysis of the chemokine receptor CCR3 on airway epithelial cells / J Immunol 177 : 3344 ~ 3354
Bell AC / 2000 / Methylation of a CTCF-dependent boundary controls imprinted expression of the Igf2 gene / Nature 405 : 482 ~ 485
Broske AM / 2009 / DNA methylation protects hematopoietic stem cell multipotency from myeloerythroid restriction / Nat Genet 41 : 1207 ~ 1215
Bystrom J / 2004 / Gene microarray analysis reveals interleukin-5-dependent transcriptional targets in mouse bone marrow / Blood 103 : 868 ~ 877
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