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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 387 - 393 (7page)
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Although mounting evidence indicates the involvement of galectin-3 in cancer progression and metastasis,the underlying molecular mechanisms remain largely unknown. In this study, we investigated the effect and possible mechanism of galectin-3 on the migration and invasion of B16F10, a metastatic melanoma cell line, in which galectin-3 and matrix metalloproteinase-1 (MMP-1) were both found to be highly expressed. Knockdown of galectin-3 with specific siRNA reduced migration and invasion, which was associated with reduced expression of MMP-1. To further investigate the underlying mechanism, we examined the effect of galectin-3 knockdown on the activity of AP-1, a transcriptional factor regulating MMP-1expression. We found that galectin-3 directly interacted with AP-1 and facilitated the binding of this complex to the MMP-1 promoter that drives MMP-1transcription. Moreover, silencing of galectin-3 inhibited binding of fra-1 and c-Jun to promoter sites of MMP-1 gene. Consistent with these in vitro findings,our in vivo study demonstrated that galectin-3 shRNA treatment significantly reduced the total number of mouse lung metastatic nodules. Taken together, galectin-3 facilitates cell migration and invasion in melanoma in vitro and can induce metastasis in vivo, in part through, regulating the transcription activity of AP-1and thereby up-regulating MMP-1 expression.
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참고문헌 (23)
참고문헌 신청
Ahonen M / 1998 / Adenovirus-mediated gene delivery of tissue inhibitor of metalloproteinases-3 inhibits invasion and induces apoptosis in melanoma cells / Cancer Res 58 : 23
Angel P / 1991 / The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation / Biochim Biophys Acta 1072 : 129 ~ 157
Benbow U / 1999 / A novel host/tumor cell interaction activates matrix metalloproteinase 1 and mediates invasion through type I collagen / J Biol Chem 274 : 25371 ~ 25378
Cheong TC / 2010 / Silencing of galectin-3 changes the gene expression and augments the sensitivity of gastric cancer cells to chemotherapeutic agents / Cancer Sci 101 : 94 ~
Durko M / 1997 / Suppression of basement membrane type IV collagen degradation and cell invasion in human melanoma cells expressing an antisense RNA for MMP-1 / Biochim Bioph
Angel P / 1991 / The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation / Biochim Biophys Acta 1072 : 129 ~ 157
Benbow U / 1999 / A novel host/tumor cell interaction activates matrix metalloproteinase 1 and mediates invasion through type I collagen / J Biol Chem 274 : 25371 ~ 25378
Cheong TC / 2010 / Silencing of galectin-3 changes the gene expression and augments the sensitivity of gastric cancer cells to chemotherapeutic agents / Cancer Sci 101 : 94 ~
Durko M / 1997 / Suppression of basement membrane type IV collagen degradation and cell invasion in human melanoma cells expressing an antisense RNA for MMP-1 / Biochim Bioph
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