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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제7호
- 발행연도
- 2006.1
- 수록면
- 1,158 - 1,162 (5page)
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초록· 키워드
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The glyoxylate cycle can conserve carbons andadequately supply tricarboxylic acid (TCA) cycle intermediates2 carbonsources. It has been reported that isocitrate lyase (ICL1), akey enzyme of the glyoxylate cycle, is highly induced whenMagnaporthe grisea, the causal agent of rice blast, infects itshost. Therefore, the glyoxylate cycle is considered as a newtarget for antifungal agents. A 1.6-kb DNA fragment encodingthe ICL1 from M. grisea KJ201 was amplified by PCR,cloned into a vector providing His-tag at the N-terminus,expressed in Escherichia coli, and purified using Ni-NTAaffinity chromatography. The molecular mas of the purifiedICL1 was approximately 60 kDa, as determined by SDS-PAGE. The ICL1 inhibitory effects of TCA cycle intermediatesand their analogs were investigated. Among them, 3-nitropropionate was found to be the strongest inhibitor withan IC50 value of 11.0g/ml. 3-Nitropropionate inhibited theappressorium development in M. grisea at the M level,also inhibited the mycelial growth of the fungus on minimalmedium containing acetate as a C2 carbon source. These resultssuggest that ICL1 plays a crucial role in apresoriumformation of M. grisea and is a new target for the control ofphytopathogenic fungal infection.
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참고문헌 (22)
참고문헌 신청
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DO, JIN HWAN / 2004 / Inference of Aspergillus fumigatus Pathways by Computational GenomeAnalysis: Tricarboxylic Acid Cycle (TCA) and Glyoxylate Shunt / Journal of Microbiolo
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Gainey, L. D. S / 1992 / Characterization of the glyoxysomal isocitrate lyase genes of Aspergillus nidulans (acuD) and Neurospora crassa (acu3) / Curr. Genet. 21 : 43 ~ 47
DO, JIN HWAN / 2004 / Inference of Aspergillus fumigatus Pathways by Computational GenomeAnalysis: Tricarboxylic Acid Cycle (TCA) and Glyoxylate Shunt / Journal of Microbiolo
Eckhard, T / 1997 / Glisoprenin A, an inhibitor of the signal transduction pathway leading to appressorium formation in germinating conidia of Magnaporthe grisea on hydrophob
Eilbert, F / 2000 / Neobulgarones A-F from cultures of Neobulgaria pura, new inhibitors of appressorium formation of Magnaporthe grisea / J. Antibiot. 53 : 1123 ~ 1129
Gainey, L. D. S / 1992 / Characterization of the glyoxysomal isocitrate lyase genes of Aspergillus nidulans (acuD) and Neurospora crassa (acu3) / Curr. Genet. 21 : 43 ~ 47
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