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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제17권 제5호
- 발행연도
- 2007.1
- 수록면
- 800 - 805 (6page)
이용수
초록· 키워드
오류제보하기
Two endoglucanases with processive celulaseactivities, produced from Fomitopsis palustrismicrocrystalline cellulose (Avicel), were purified to homogeneityby anion-exchange and gel filtration column chromatographysystems. SDS-PAGE analysis indicated that the molecularmases of the purified enzymes were 47 kDa and 35 kDa,respectively. The amino acid sequence analysis of the 47-kDaprotein (EG47) showed a sequence similarity with fungalglycoside hydrolase family 5 endoglucanase from the white-rot fungus Phanerochaete chrysosporium. N-terminal andhowever, had no homology with any other glycosylhydrolases,although the enzyme had high specific activity againstcarboxymethyl cellulose, which is a typical substrate forendoglucanases. The initial rate of Avicel hydrolysis by EG35was relatively fast for 48 h, and the amount of soluble reducingsugar released after 96 h was 100g/ml. Although EG47also hydrolyzed Avicel, the hydrolysis rate was lower thanthat of EG35. Thin layer chromatography analysis of thehydrolysis products released from Avicel indicated that thefungus possesses processive EGs capable of degradingcrystalline cellulose.
목차
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참고문헌 (27)
참고문헌 신청
/ 1987 / Enzymatic hydrolysis of cellulose Is the current theory of mechanism of hydrolysis valid : 67 ~ 87
Bradford / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding : 248 ~ 254
Chen.H.-G / 2006 / Purification and characterization of nevel bifunctional xylanase, XynIII, isolated from Aspergillus niger A-25 16 : 1132 ~ 1138
Cohen, R / 2005 / Processive endoglucanase active in crystalline cellulose hydrolysis by the brown rot basidiomycete Gloeophyllum trabeum 71 : 2412 ~ 2417
Eriksson / 1990 / Microbial and Enzymatic Degradation of Wood and Wood Components
Bradford / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding : 248 ~ 254
Chen.H.-G / 2006 / Purification and characterization of nevel bifunctional xylanase, XynIII, isolated from Aspergillus niger A-25 16 : 1132 ~ 1138
Cohen, R / 2005 / Processive endoglucanase active in crystalline cellulose hydrolysis by the brown rot basidiomycete Gloeophyllum trabeum 71 : 2412 ~ 2417
Eriksson / 1990 / Microbial and Enzymatic Degradation of Wood and Wood Components
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Functional Analysis of a Gene Encoding Endoglucanase that Belongs toGlycosyl Hydrolase Family 12 from the Brown-Rot Basidiomycete Fomitopsis palustris
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Identification of a Gene Encoding Endoglucanase That Belongs to Glycosyl Hydrolase Family 45 from the Brown-Rot Basidiomycete Fomitopsis palustris
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일본에서의 최근의 셀룰로오스의 연구동향 -제2회 일본셀룰로오스학회 ('95 Cellulose R ＆ D)에 참가하여-
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Biosynthesis of cellulose
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Cellulose Hydrolysis by Digestive Enzymes of Reticulitermes speratus, a Native Termite from Korea
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소양호에서 Cellulose Film의 분해와 부착 세균의 분포 변화
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Activities of Extracellular Enzymes from White-rot Fungi, Phanerochaete chrysosporium
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