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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 163 - 167 (5page)
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Since D-xylose is not fermentable in Saccharomyces cerevisiae, its conversion to D-xylulose is required for its application in biotechnological industries using S. cerevisiae. In order to convert D-xylose to D-xylulose by way of an enzyme immobilized system, D-xylose isomerase (XI) of Escherichia coli was fused with 10-arginine tag (R10) at its C-terminus for the simple purification and immobilization process using a cation exchanger. The fusion protein XIR10 was overexpressed in recombinant E. coli and purified to a high purity by a single step of cation exchange chromatography. The purified XIR10 was immobilized to a cation exchanger via the electrostatic interaction with the C-terminal 10-arginine tag. Both the free and immobilized XIR10 exhibited similar XI activities at various pH values and temperatures, indicating that the immobilization to the cation exchanger has a small effect on the enzymatic function of XIR10. Under optimized conditions for the immobilized XIR10, Dxylose was isomerized to D-xylulose with a conversion yield of 25%. Therefore, the results of this study clearly demonstrate that the electrostatic immobilization of XIR10 via the interaction between the 10-arginine tag and a cation exchanger is an applicable form of the conversion of D-xylose to D-xylulose.
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참고문헌 (18)
참고문헌 신청
Brewer, S. J / 1985 / The purification of recombinant proteins using C-terminal polyarginine fusions / Trends Biotechnol 3 : 119 ~ 122
Chandrakant, P / 2000 / Application of a compatible xylose isomerase in simultaneous bioconversion of glucose and xylose to ethanol / Biotechnol. Bioprocess Eng 5 : 32 ~ 39
Farber, G. K / 1989 / Crystallographic studies of the mechanism of xylose isomerase / Biochemistry 28 : 7289 ~ 7297
Gong, C / 1981 / Direct fermentation of D-xylose to ethanol by a xylose-fermentating yeast mutant, Candida sp. XF 217 / Biotechnol. Lett 3 : 245 ~ 250
Jokela, J. / 2002 / Isomerization of pentose and hexose sugars by an enzyme reactor packed with cross-linked xylose isomerase crystals / Enzyme Microb. Technol 31 : 67 ~ 76
Chandrakant, P / 2000 / Application of a compatible xylose isomerase in simultaneous bioconversion of glucose and xylose to ethanol / Biotechnol. Bioprocess Eng 5 : 32 ~ 39
Farber, G. K / 1989 / Crystallographic studies of the mechanism of xylose isomerase / Biochemistry 28 : 7289 ~ 7297
Gong, C / 1981 / Direct fermentation of D-xylose to ethanol by a xylose-fermentating yeast mutant, Candida sp. XF 217 / Biotechnol. Lett 3 : 245 ~ 250
Jokela, J. / 2002 / Isomerization of pentose and hexose sugars by an enzyme reactor packed with cross-linked xylose isomerase crystals / Enzyme Microb. Technol 31 : 67 ~ 76
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