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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 209 - 214 (6page)
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VBNC (Viable but nonculturable) state is an adaptive response of cells in adverse environments, which lead cell not grow on routine nutrient agar. In this study, we induced VBNC in Escherichia coli using copper and verify the characterization of it. After treatment of copper, we didn’ detect any cells via plate cultivation, namely, colony forming unit (CFU) was zero. However, we identified the existence of VBNC by staining live cells with Live/Dead BacLight bacterial viability kit and counting them through flow cytometry. Then we isolated genomic DNA and RNA from VBNC-induced cells and analyzed the stability of them. Degradation of RNA is more severe than that of DNA and RNA is degraded as specific fragments. In addition, we showed the morphology of VBNC cell by Bio-Transmission Electron Microscope (Bio-TEM). VBNC cell showed impaired periplasmic space and inner and outer membrane were separated and the amount of cytosol were significantly decreased
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참고문헌 (21)
참고문헌 신청
Alexander, E. / 1999 / The viable-but-nonculturable condition is induced by copper in Agrobacterium tumefaciens and Rhizobium leguminosarum / Appl. Environ. Microbiol 65 : 37
Arana, I. / 2007 / Inability of Escherichia coli to resuscitate from the viable but nonculturable state / FEMS Microbiol. Ecol 62 : 1 ~ 11
Ashley P. Day / 2004 / Changes in Membrane Fatty Acid Composition during Entry of Vibrio vulnificus into the Viable But Nonculturable State / The Journal of Microbiology 42 (
Beumer, R. R. / 1992 / Campylobacter jejuni non-culturable coccoid cells / Int. J. Food Microbiol 15 : 153 ~ 163
Grey, B. / 2001 / Concentrations of copper thought to be toxic to Escherichia coli can induce the viable but nonculturable condition / Appl. Environ. Microbiol 67 : 5325 ~ 53
Arana, I. / 2007 / Inability of Escherichia coli to resuscitate from the viable but nonculturable state / FEMS Microbiol. Ecol 62 : 1 ~ 11
Ashley P. Day / 2004 / Changes in Membrane Fatty Acid Composition during Entry of Vibrio vulnificus into the Viable But Nonculturable State / The Journal of Microbiology 42 (
Beumer, R. R. / 1992 / Campylobacter jejuni non-culturable coccoid cells / Int. J. Food Microbiol 15 : 153 ~ 163
Grey, B. / 2001 / Concentrations of copper thought to be toxic to Escherichia coli can induce the viable but nonculturable condition / Appl. Environ. Microbiol 67 : 5325 ~ 53
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