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Penicillium oxalicum strain CBPS-3F-Tsa, anefficient phosphate solubilizing fungus, was evaluated for itsproduction of organic acid in vitro and effect of inoculation onthe growth promotion of Maize under greenhouse conditions.The fungus solubilized 129.1, 118.8, and 54.1 mg P/l of tricalciumphosphate [Ca3(PO4)2], aluminum phosphate (AlPO4),and ferric phosphate (FePO4), respectively, after 72 h ofincubation. Malic acid, gluconic acid, and oxalic acid weredetected in the flasks supplemented with various phosphatesources [240, 146, 145 mM/l AlPO4, FePO4, and Ca3(PO4)2,respectively] together with a large amount of malic acidfollowed by the other two. The effects of inoculation of P.oxalicum CBPS-3F-Tsa on maize plants were studied underpot culture conditions. P. oxalicum CBPS-3F-Tsa was inoculatedto maize plants alone or together with inorganic phosphates inthe form of fused phosphates (FP) and rock phosphates (RP).Inoculation of P. oxalicum CBPS-3F-Tsa increased the plantgrowth and N and P accumulation in plants, compared withcontrol plants, and also had positive effects when appliedwith RP. The results of this study show that the fungusP. oxalicum strain CBPS-3F-Tsa could solubilize differentinsoluble phosphates by producing organic acids, particularlymalic acid, and also improved the efficiency of RP applied tomaize plants.

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