지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 9 - 19 (11page)
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The Bacillus subtilis pyrimidine biosynthetic (pyr) operon encodes all of the enzymes for the de novo biosynthesis of Uridine monophosphate (UMP) and additional cistrones encoding a uracil permease and the regulatory protein PyrR. The PyrR is a bifunctional protein with pyr mRNA-binding regulatory funtion and uracil phosphoribosyltransferase activity. To study the global regulation by the pyrR deletion, the proteome comparison between Bacillus subtilis DB104 and Bacillus subtilis DB104 ΔpyrR in the minimal medium without pyrimidines was employed. Proteome analysis of the cytosolic proteins from both strains by 2D-gel electrophoresis showed the variations in levels of protein expression. On the silver stained 2D-gel with an isoelectric point (pI) between 4 and 10, about 1,300 spots were detected and 172 spots showed quantitative variations in which 42 high quantitatively variant proteins were identified. The results showed that production of the pyrimidine biosynthetic enzymes (PyrAA, PyrAB, PyrB, PyrC, PyrD, and PyrF) were significantly increased in B. subtilis DB104 ΔpyrR. Besides, proteins associated carbohydrate metabolism, elongation protein synthesis, metabolism of cofactors and vitamins, motility, tRNA synthetase, catalase, ATP–binding protein, and cell division protein FtsZ were overproduced in the PyrR–deficient mutant. Based on analytic results, the PyrR might be involved a number of other metabolisms or various phenomena in the bacterial cell besides the pyrimidine biosynthesis.
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참고문헌 (21)
참고문헌 신청
Aldritt, S. M. / 1985 / Pyrimidine salvagein Giardia lamblia / J. Exp. Med 161 : 437 ~ 445
Bonner, E. R. / 2001 / Molecular recognition of pyr mRNA by the Bacillussubtilis attenuation regulatory protein PyrR / Nucleic AcidsRes 29 : 4851 ~ 4865
Bradford, M. M / 1976 / A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein-dye-binding / Anal. Biochem 72 :
Chander, P. / 2005 / Structure ofthe nucleotide complex of PyrR, the pyr Attenuation proteinfrom Bacillus caldolyticus, suggests dual regulation by pyrimidineand purine nucle
Fields, C. J / 2007 / Regulation of pyr geneexpression in Mycobacterium smegmatis by PyrR-dependenttranslational repression / J. Bacteriol 189 : 6236 ~ 6245
Bonner, E. R. / 2001 / Molecular recognition of pyr mRNA by the Bacillussubtilis attenuation regulatory protein PyrR / Nucleic AcidsRes 29 : 4851 ~ 4865
Bradford, M. M / 1976 / A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein-dye-binding / Anal. Biochem 72 :
Chander, P. / 2005 / Structure ofthe nucleotide complex of PyrR, the pyr Attenuation proteinfrom Bacillus caldolyticus, suggests dual regulation by pyrimidineand purine nucle
Fields, C. J / 2007 / Regulation of pyr geneexpression in Mycobacterium smegmatis by PyrR-dependenttranslational repression / J. Bacteriol 189 : 6236 ~ 6245
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