지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제8호
- 발행연도
- 2008.1
- 수록면
- 1,377 - 1,385 (9page)
이용수
초록· 키워드
오류제보하기
The uracil-sensing domain in archaeal family B-type DNA
polymerases recognizes pro-mutagenic uracils in the DNA
template, leading to stalling of DNA polymerases. Here,
we describe our new findings regarding the molecular
mechanism underpinning the stalling of polymerases. We
observed that two successive deaminated bases were required
to stall TNA1 and KOD1 DNA polymerases, whereas a
single deaminated base was enough for stalling Pfu DNA
polymerase, in spite of the virtually identical uracil-sensing
domains. TNA1 and KOD1 DNA polymerases have a much
higher extension rate than Pfu DNA polymerase; decreasing
the extension rate resulted in stalling by TNA1 and KOD1
DNA polymerases at a single deaminated base. These results
strongly suggest that these polymerases require two factors
to stop DNA polymerization at a single deaminated base:
the presence of the uracil-sensing domain and a relatively
slow extension rate.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
BAE, SEUNG SEOB / 2006 / Thermococcus onnurineus sp. nov., a Hyperthermophilic Archaeon Isolatedfrom a Deep-Sea Hydrothermal Vent Area at the PACMANUS Field / Journal of Micr
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal / Biochem 7
Cann, I. K. / 1999 / Archaeal DNA replication: Identifying the pieces to solve a puzzle / Genetics 152 : 1249 ~ 1267
Cho, Y. / 2007 / Characterization of a dUTPase from the hyperthermophilic archaeon Thermococcus onnurineus NA1 and its application in polymerase chain reaction amplification.
Chung, J. H. / 2001 / Biochemical characterization of a novel hypoxanthine/xanthine dNTP pyrophosphatase from Methanococcus jannaschii / Nucleic Acids Res 29 : 3099 ~ 3107
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal / Biochem 7
Cann, I. K. / 1999 / Archaeal DNA replication: Identifying the pieces to solve a puzzle / Genetics 152 : 1249 ~ 1267
Cho, Y. / 2007 / Characterization of a dUTPase from the hyperthermophilic archaeon Thermococcus onnurineus NA1 and its application in polymerase chain reaction amplification.
Chung, J. H. / 2001 / Biochemical characterization of a novel hypoxanthine/xanthine dNTP pyrophosphatase from Methanococcus jannaschii / Nucleic Acids Res 29 : 3099 ~ 3107
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
DNA Polymerase 의 구조 및 기능 연구 - DNA 염기서열 결정에 응용 - ( Structural and Functional Aspects of DNA Polymerase - Applications to DNA Sequencing - )
생명과학
1993 .10
-
Methods for Extraction of DNA from Water Samples for Polymerase Chain Reaction
The Journal of Microbiology
1997 .12
-
Cloning, Purification, and Characterization of a New DNA Polymerase froma Hyperthermophilic Archaeon, Thermococcus sp. NA1
Journal of Microbiology and Biotechnology
2007 .01
-
Fabrication of DNA Microarrays Utilizing Surface Polymerase Reactions
한국분석과학회 학술대회
2009 .05
-
PCR을 이용한 인간 세포거대바이러스(HCMV) DNA 중합효소 유전자의 검색
The Journal of the Korean Society for Microbiology
1992 .04
-
Excellent DNA Polymerase from Thermococcus kodakaraensis KOD1 and Its Application for PCR
한국미생물학회 학술대회논문집
2010 .05
-
The Invariant Region I Sequence of the Adenovirus Serotype 2 DNA Polymerase Influences Template Specificity during DNA Synthesis
The Journal of Microbiology
1998 .09
-
Thermus thermophilus HJ6 유래 N-말단 결실 DNA Polymerase의 정제 및 특성
한국미생물·생명공학회지
2010 .01
-
Detection of methylated DNA with a polymerase by atomic force microscopy
한국진공학회 학술발표회초록집
2016 .08
-
Structural Analysis of Ligand Bound Complex of UdgX : A Unique Uracil DNA Glycosylase Superfamily Binding Uracil DNA
한국미생물학회 학술대회논문집
2016 .04
-
제한효소 처리된 Genomic DNA에 의한 Polymerase Chain Reaction 증폭 효율에 관한 연구
미생물학회지
2004 .09
-
Regulation of Bacterial Transcription Initiation by Factors that Bind Directly to RNA Polymerase and Not to DNA
한국미생물학회 학술대회논문집
2019 .04
-
Overexpression of the Bacteriophage PRD1 DNA Polymerase
미생물학회지
1992 .04
-
Relationship Between Genome Similarity and DNA-DNA Hybridization Among Closely Related Bacteria
Journal of Microbiology and Biotechnology
2007 .01
-
Role of RNA Polymerase II Carboxy Terminal Domain Phosphorylation in DNA Damage Response
The Journal of Microbiology
2005 .12
-
DNA-based information storage
한국진공학회 학술발표회초록집
2017 .02
-
Cloning, Expression, and Characterization of DNA Polymerase fromHyperthermophilic Bacterium Aquifex pyrophilus
Journal of Microbiology and Biotechnology
2004 .01
-
Site-specific Mutagenesis에 의한 PRD1 DNA Polymerase의 활성부위 결정
미생물학회지
1991 .01
-
PCR을 이용한 결핵균 진단에 있어서 효과적인 DNA 추출법
The Journal of the Korean Society for Microbiology
1994 .04
-
Direct Extraction of DNA from Soil for Amplification of 16S rRNA Gene Sequences by Polymerase Chain Reaction
The Journal of Microbiology
1996 .09
이 논문의 저자 정보
최근 본 자료
댓글(0)
0