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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제8호
- 발행연도
- 2011.1
- 수록면
- 802 - 807 (6page)
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초록· 키워드
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Cationic liposomes have been actively used as gene delivery vehicle because of their minimal toxicity, but their relatively low efficiency of gene delivery is the major disadvantage of these vectors. Recently, cysteine residue incorporation to HIV-1 Tat peptide increased liposomemediated transfection compared with unmodified Tat peptide. Therefore, we designed a novel modified Tat peptide having a homodimeric (Tat-CTHD, Tat-NTHD)and closed structure (cyclic Tat) simply by using the disulfide bond between cysteines to develop a more efficient and safe nonviral gene delivery system. The mixing of Tat-CTHD and Tat-NTHD with DNA before mixing with lipofectamine increased the transfection efficiency compared with unmodified Tat peptide and lipofectamine only in MCF-7 breast cancer cells and rat vascular smooth muscle cells. However, cyclic Tat did not show any improvement in the transfection efficiency. In the gel retardation assay, Tat-CTHD and Tat-NTHD showed more strong binding with DNA than unmodified Tat and cyclic Tat peptide. This enhancement was only shown when Tat-CTHD and Tat-NTHD were mixed with DNA before mixing with lipofectamine. The effects of Tat-CTHD and Tat-NTHD were also valid in the experiment using DOTAP and DMRIE instead of lipofectamine. We could not find any significant cytotoxicity in the working concentration and more usage of these peptides. In conclusion, we have designed a novel transfection-enhancing peptide by easy homodimerization of Tat peptide, and the simple mix of these novel peptides with DNA increased the gene transfer of cationic lipids more efficiently with no additional cytotoxicity.
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참고문헌 (24)
참고문헌 신청
Anwer, K / 2010 / Phase-I clinical trial of IL-12 plasmid/lipopolymer complexes for the treatment of recurrent ovarian cancer / Gene Ther 17 : 360 ~ 369
Astriab-Fisher, A / 2002 / Conjugates of antisense oligonucleotides with the Tat and antennapedia cell-penetrating peptides: Effects on cellular uptake, binding to target seq
Bulaj, G. / 1998 / Ionizationreactivity relationships for cysteine thiols in polypeptides / Biochemistry 37 : 8965 ~ 8972
Cheng, S / 1994 / Design and synthesis of novel cyclic RGD-containing peptides as highly potent and selective integrin alpha IIb beta 3 antagonists / J. Med. Chem 37 : 1 ~ 8
Elmquist, A. / 2001 / VE-cadherin-derived cell-penetrating peptide, pVEC, with carrier functions / Exp. Cell Res 269 : 237 ~ 244
Astriab-Fisher, A / 2002 / Conjugates of antisense oligonucleotides with the Tat and antennapedia cell-penetrating peptides: Effects on cellular uptake, binding to target seq
Bulaj, G. / 1998 / Ionizationreactivity relationships for cysteine thiols in polypeptides / Biochemistry 37 : 8965 ~ 8972
Cheng, S / 1994 / Design and synthesis of novel cyclic RGD-containing peptides as highly potent and selective integrin alpha IIb beta 3 antagonists / J. Med. Chem 37 : 1 ~ 8
Elmquist, A. / 2001 / VE-cadherin-derived cell-penetrating peptide, pVEC, with carrier functions / Exp. Cell Res 269 : 237 ~ 244
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