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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제3호
- 발행연도
- 2009.1
- 수록면
- 271 - 276 (6page)
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초록· 키워드
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Functional gene studies in the cultivated white button
mushroom Agaricus bisporus have been constrained by the
absence of effective gene-silencing tools. Using two endogenous
genes from A. bisporus, we have tested the utility of dsRNA
hairpin constructs to mediate downregulation of specific
genes. Hairpin constructs for genes encoding orotidine 5'-
monophosphate decarboxylase (URA3) and carboxin resistance
(CBX) were introduced into A. bisporus using Agrobacteriummediated
transfection. Although predicted changes in
phenotype were not observed in vitro, quantitative-PCR
analyses indicated unambiguously that transcripts in several
transformants were substantially reduced compared with
the non-transformed controls. Interestingly, some hairpin
transformants exhibited increased transcription of target
genes. Our observations show that hairpin transgenic
sequences can mediate downregulation of A. bisporus
endogenous genes and that the technology has the potential
to expedite functional genomics of the mushroom.
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참고문헌 (24)
참고문헌 신청
Bundock, P. / 1999 / T-DNA from Agrobacterium tumefaciens as an efficient tool for gene targeting in Kluyveromyces lactis / Mol. Gen. Genet 261 : 115 ~ 121
Burns, C. / 2005 / Efficient GFP expression in the mushroom Agaricus bisporus and Coprinus cinereus requires introns / Fungal Genet. Biol 42 : 191 ~ 199
Burns, C. / 2006 / Evaluation of Agrobacteriummediated transformation of Agaricus bisporus using a range of promoters linked to hygromycin resistance / Mol. Biotechnol 32 : 1
Challen, M. / 1987 / Production and evaluation of fungicide resistant mutants in the cultivated mushroom Agaricus bisporus / Trans. Br. Mycol. Soc 88 : 433 ~ 439
Challen, M. P. / 1996 / Molecular cloning and characterization of the Agaricus bisporus TRP2 gene. in: Mushroom Biology and Mushroom Products / Penn State : 47 ~ 56
Burns, C. / 2005 / Efficient GFP expression in the mushroom Agaricus bisporus and Coprinus cinereus requires introns / Fungal Genet. Biol 42 : 191 ~ 199
Burns, C. / 2006 / Evaluation of Agrobacteriummediated transformation of Agaricus bisporus using a range of promoters linked to hygromycin resistance / Mol. Biotechnol 32 : 1
Challen, M. / 1987 / Production and evaluation of fungicide resistant mutants in the cultivated mushroom Agaricus bisporus / Trans. Br. Mycol. Soc 88 : 433 ~ 439
Challen, M. P. / 1996 / Molecular cloning and characterization of the Agaricus bisporus TRP2 gene. in: Mushroom Biology and Mushroom Products / Penn State : 47 ~ 56
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