지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 393 - 401 (9page)
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초록· 키워드
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A bacterium producing antimicrobial substance was isolated from cheonggukjang. The bacterium was identified as a strain of Bacillus subtilis by 16S rDNA sequencing and designated as Bacillus subtilis HH28. The antimicrobial substance produced from Bacillus subtilis HH28 was purified by 0-80% ammonium sulfate precipitation, DEAE-sepharose FF column chromatography, and Sephacryl S-200 HR gel chromatography. The molecular weight of the purified antimicrobial substance was estimated to be approximately 3,500 Da using Tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis and direct detection analysis. Antimicrobial substance from B. subtilis HH28 not only inhibited B. cereus, but also Listeria monocytogenes and Vibrio parahaemolyticus. The purified antimicrobial substance was stable at 40-80oC, and between pH 2 and 8. Antimicrobial activity of the purified substance was completely destroyed by treatment of protease, proteinase K, and pronase E, indicating that it is proteinaceous.
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참고문헌 (24)
참고문헌 신청
Abriouel H / 2011 / Diversity and applications of Bacillus bacteriocins / FEMS Microbiol. Rev 35 : 201 ~ 232
Andersson A / 1995 / What problems does the food industry have with the spore-forming pathogens Bacillus cereus and Clostridium perfringens? / Int. J. Food Microbiol 28 : 45
Bhunia AK / 1987 / Direct detection of an antimicrobial peptide of Pediococcus acidilactici in sodium dodecyl sulfate-polyacrylamide gel electrophoresis / J. Ind. Microbiol 2
Edman P / 1967 / A protein sequenator / Eur. J. Biochem 1 : 80 ~ 91
He Z / 2007 / Isolation and identification of a Paenibacillus polymyxa strain that coproduces a novel lantibiotic and polymyxin / Appl. Environ. Microbiol 73 : 168 ~ 178
Andersson A / 1995 / What problems does the food industry have with the spore-forming pathogens Bacillus cereus and Clostridium perfringens? / Int. J. Food Microbiol 28 : 45
Bhunia AK / 1987 / Direct detection of an antimicrobial peptide of Pediococcus acidilactici in sodium dodecyl sulfate-polyacrylamide gel electrophoresis / J. Ind. Microbiol 2
Edman P / 1967 / A protein sequenator / Eur. J. Biochem 1 : 80 ~ 91
He Z / 2007 / Isolation and identification of a Paenibacillus polymyxa strain that coproduces a novel lantibiotic and polymyxin / Appl. Environ. Microbiol 73 : 168 ~ 178
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