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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 339 - 346 (8page)
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A bacterial strain was isolated from homemade doenjang (Korean fermented soybean paste) as a producer of the extracellular β-galactosidase, capable of hydrolyzing lactose to liberate galactose and glucose residues. The isolate YB-1414 has been identified as Bacillus licheniformis on the basis of its 16S rDNA sequence, morphology and biochemical properties. The production of β-galactosidase by B. licheniformis YB-1414 reached maximum levels of 6.2 U/ml in culture medium containing wheat bran (1%) and yeast extract (2.5%) as carbon and nitrogen sources, respectively. Particularly, the insoluble fraction was more effective for β-galactosidase production than the soluble extract of wheat bran. The enzyme exhibited maximum activity for hydrolysis of para-nitrophenyl-β-D-galactopyranoside (pNP-βGal) under reaction conditions of pH 6.0 and 55-60oC. Its hydrolyzing activity for pNP-βGal was drastically decreased by the addition of low concentrations of galactose, but only slightly decreased by glucose, with 85% of maximal activity in the presence of 400 mM glucose.
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참고문헌 (22)
참고문헌 신청
Batra N / 2002 / Production and characterization of a thermostable β-galactosidase from Bacillus coagulans RCS3 / Biotechnol. Appl. Biochem 36 : 1 ~ 6
Batra N / 2011 / Applications of β-gal-III isozyme from Bacillus coagulans RCS3, in lactose hydrolysis / Int. J. Biol. Macromol 49 : 879 ~ 884
Chen W / 2008 / Production, purification, and characterization of a potential thermostable galactosidase for milk lactose hydrolysis from Bacillus stearothermophilus / J. Dai
Chen W / 2009 / Immobilization of recombinant thermostable β-galactosidase from Bacillus stearothermophilus for lactose hydrolysis in milk / J. Dairy Sci 92 : 491 ~ 498
Dong Y-N / 2011 / Enhancement of the hydrolysis activity of β-galactosidase from Geobacillus stearothermophilus by saturation mutagenesis / J. Dairy Sci 94 : 1176 ~ 1184
Batra N / 2011 / Applications of β-gal-III isozyme from Bacillus coagulans RCS3, in lactose hydrolysis / Int. J. Biol. Macromol 49 : 879 ~ 884
Chen W / 2008 / Production, purification, and characterization of a potential thermostable galactosidase for milk lactose hydrolysis from Bacillus stearothermophilus / J. Dai
Chen W / 2009 / Immobilization of recombinant thermostable β-galactosidase from Bacillus stearothermophilus for lactose hydrolysis in milk / J. Dairy Sci 92 : 491 ~ 498
Dong Y-N / 2011 / Enhancement of the hydrolysis activity of β-galactosidase from Geobacillus stearothermophilus by saturation mutagenesis / J. Dairy Sci 94 : 1176 ~ 1184
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