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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 343 - 353 (11page)
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초록· 키워드
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In the present study, the isolation of selenium (Se)-enriched bacteria from rumen fluid and hot spring water was carried out. Rumen fluid samples were taken from cannulated goats fed a basal diet and the water samples were collected from Selayang hot spring, Selangor- Malaysia. A total number of 140 Se-tolerant isolates were obtained aerobically using an Se-enriched medium and spread plate technique. All the isolates were initially screened for the ability to transform the Se-containing medium to a red-orange culture using a spectrophotometer. Twenty isolates of dark red-orange medium were selected for a screening of the highest Se-containing protein accumulating strains using the dialysis technique and icp.ms to measure the Se content. Four isolates, identified as Enterobacter cloacae (ADS1, ADS7, and ADS11), and Klebsiella pneumoniae (ADS2) from rumen fluid origin, as well as, one isolate from hot spring water (Stenotrophomonas maltophilia (ADS18)), were associated with the highest biomass organic Se-containing protein when grown in a medium enriched with 10 μg/ml sodium selenite. In addition, around 50 μg/100 μg of the absorbed inorganic Se was accumulated as an organic form. Organic Se-containing protein in all the selected strains showed antioxidant properties in the range of 0.306 to 0.353 Trolox equivalent antioxidant capacity (TEAC) mg/ml. Therefore, these strains may offer a potential source of organic Se due to their Se-tolerant nature and higher biomass organic to inorganic Se ratio.
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참고문헌 (24)
참고문헌 신청
Andreoni V / 2000 / Selenite tolerance and accumulation in the Lactobacillus species / Ann. Microbiol 50 : 77 ~ 88
Antonioli P / 2007 / Stenotrophomonas maltophilia SeITE02, a new bacterial strain suitable for bioremediation of selenite-contaminated environmental matrices / Appl. Environ
Chapman J / 2010 / Period four metal nanoparticles on the inhibition of biofouling / Colloids Surf. B Biointerfaces 78 : 208 ~ 216
Chen L / 2005 / Protective Effect of selenium-enriched Lactobacillus on CCl-4-induced liver injury in mice and its possible mechanisms / World J. Gastroenterol 11 : 5795 ~ 5
Dong Y / 2003 / Delineation of the molecular basis for selenium-induced growth arrest in human prostate cancer cells by oligonucleotide array / Cancer Res 63 : 52 ~ 59
Antonioli P / 2007 / Stenotrophomonas maltophilia SeITE02, a new bacterial strain suitable for bioremediation of selenite-contaminated environmental matrices / Appl. Environ
Chapman J / 2010 / Period four metal nanoparticles on the inhibition of biofouling / Colloids Surf. B Biointerfaces 78 : 208 ~ 216
Chen L / 2005 / Protective Effect of selenium-enriched Lactobacillus on CCl-4-induced liver injury in mice and its possible mechanisms / World J. Gastroenterol 11 : 5795 ~ 5
Dong Y / 2003 / Delineation of the molecular basis for selenium-induced growth arrest in human prostate cancer cells by oligonucleotide array / Cancer Res 63 : 52 ~ 59
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