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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제1호
- 발행연도
- 2005.1
- 수록면
- 91 - 98 (8page)
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The hyoscyamine 6β-hydroxylase (h6h) gene wasintroduced into the genome of Scopolia parviflora through theAgrobacterium rhizogenes binary vector system. The enzymewas expressed ally and tissue specific selectively in roots,resulting in five transgenic hairy root lines. The presence ofthe h6h gene in kanamycin-resistant hairy roots and itsoverexpression were confirmed by polymerase chain reaction(PCR), Northern blotting, and Western blotting, respectively.In the transgenic hairy root lines which constitutively expressedthe H6H enzyme, hyoscyamine and scopolamine accumulatedin high concentration. Among the transgenic hairy root lines thatexpressed the H6H enzyme, only two were more productive.The levels of tropane alkaloids in transgenic hairy root variedgreatly: The best transgenic line (#5) contained 8.12 mg ofscopolamine per g dry weight, which produced the compoundthree times more than wild-type root. These results suggest apossibility of improving the yield of tropane alkaloids in hairyroot lines by genetic and metabolic engineering.
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참고문헌 (18)
참고문헌 신청
Bonhomme / 2000 / Effects of the rol C gene on hairy root Inductiondevelopment and tropane alkaloid production by Atropabelladonna 63 : 1249 ~ 1252
Canel / 1998 / Effects of over-expression of strictosidinesynthase and tryptophan decarboxylase on alkaloid productionby cell cultures of Catharanthus roseus 2205 : 414 ~ 205
Gamberg / 1968 / Nutrientrequirements of suspension cultures of soybean root cells : 148 ~ 151
Goel / 2004 / Improved plant growth fromseed bacterization using siderophore overproducing coldresistant mutant of Pseudomonas fluorescens 14 : 653 ~ 657
Hamill / 1987 / New routes to plant secondary products : 800 ~ 804
Canel / 1998 / Effects of over-expression of strictosidinesynthase and tryptophan decarboxylase on alkaloid productionby cell cultures of Catharanthus roseus 2205 : 414 ~ 205
Gamberg / 1968 / Nutrientrequirements of suspension cultures of soybean root cells : 148 ~ 151
Goel / 2004 / Improved plant growth fromseed bacterization using siderophore overproducing coldresistant mutant of Pseudomonas fluorescens 14 : 653 ~ 657
Hamill / 1987 / New routes to plant secondary products : 800 ~ 804
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미치광이풀(Scopolia parviflora)의 모상근 배양에 의한 Tropane Alkaloid 생산
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Multi-analytical Method Development for Tropane Alkaloids (TAs) in Plant Food by LC-MS/MS
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Production of Red Ginseng Specific Ginsenosides $(Rg_2, Rg_3, Rh_1 and Rh_2)$ from Agrobacterium-transformed hairy Roots of Panax ginseng by Heat Treatment
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