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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제1호
- 발행연도
- 2005.1
- 수록면
- 105 - 111 (7page)
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초록· 키워드
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End-stage renal disease is a fatal and devastatingdisease that is caused by progressive and irreversible lossof functioning nephrons in the kidney. Dialysis and renaltransplantation are the common treatments at present, butthese treatments have severe limitations. The present studyinvestigated the possibility of reconstructing renal tissues bytransplantation of renal precursor cells to replace the currenttreatments for end-stage renal disease. Embryonic renalprecursor cells, freshly isolated from metanephroi of rat fetusat day 15 post-gestation, were seeded on biodegradable polymerscaffolds and transplanted into peritoneal cavities of athymic micefor three weeks. Histologic sections stained with hematoxylin& eosin and periodic acid-Schiff revealed the formation ofprimitive glomeruli, tubules, and blood vessels, suggestingthe potential of embryonic renal precursor cells to reconstituterenal tissues. Immunohistochemical staining for proliferatingcell nuclear antigen, a marker of proliferating cells, showedintensive nuclear expression in the regenerated renal structures,suggesting renal tissue reconstitution by transplanted embryonicrenal precursor cells. This study demonstrates the reconstitutionof renal tissue in vivo by transplanting renal precursor cellswith biodegradable polymer scaffolds, which could be utilizedas a new method for partial or full restoration of renal structureand function in the treatment of end-stage renal disease.
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참고문헌 (19)
참고문헌 신청
A / 2002 / Stem cells in thekidney 61 : 387 ~ 395
Hammerman / 2002 / Transplantation of developingkidneys 16 : 62 ~ 71
Humes / 1999 / Replacement of renal functionFig Immunohistochemical staining against PCNA Many PCNA-positive nuclei were observed in the developingprimitive tubules PCNA-posi
Kaminski / 2002 / Engraftment and differentiation of humanmetanephroi into functional mature nephrons after transplantationinto mice is accompanied by a profile of gene expre
Kim / 2000 / Principles of Tissue Engineering : 655 ~ 667
Hammerman / 2002 / Transplantation of developingkidneys 16 : 62 ~ 71
Humes / 1999 / Replacement of renal functionFig Immunohistochemical staining against PCNA Many PCNA-positive nuclei were observed in the developingprimitive tubules PCNA-posi
Kaminski / 2002 / Engraftment and differentiation of humanmetanephroi into functional mature nephrons after transplantationinto mice is accompanied by a profile of gene expre
Kim / 2000 / Principles of Tissue Engineering : 655 ~ 667
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