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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제4호
- 발행연도
- 2006.1
- 수록면
- 569 - 575 (7page)
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The 530 stem-loop is a 46 nucleotide stem-loopstructure found in all small-subunit ribosomal RNAs.requirement for Watson-Crick interactions between thenucleotides 505-507 and 524-526 (530 pseudoknot), whichare highly conserved. To examine the nature and functionalsignificance of these interactions, a random mutagenesisexperiment was conducted in which the nucleotides in theproposed pseudoknot were simultaneously mutated andfunctional mutants were selected and analyzed. Geneticposition except 524 was not exclusively critical to theselection of functional mutants. It also indicated that base-pairing interactions between the positions 505-507 and 524-526 were required for ribosomal function, and much weakerbase-pairing interactions than those of the wild-type alsoallowed high ribosomal function. Our results support thehypothesis that the 530 pseudoknot structure may undergo aduring certain stages of the protein synthesis process byinteracting with other ligands present in its environment.
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참고문헌 (23)
참고문헌 신청
Allen, P. N / 1989 / Mutations in ribosomal proteins S4 and S12 influence the higher order structure of 16S ribosomal RNA / J. Mol. Biol. 208 : 457 ~ 468
Bakin, A / 1994 / The single pseudouridine residue in Escherichia coli 16S RNA is located at position 516 / Nucleic Acids Res. 22 : 3681 ~ 3684
Bock, A / 1979 / Ribosomal ambiguity (ram) mutations facilitate diyhydrostreptomycin binding to ribosomes / FEBS Lett. 104 : 317 ~ 321
Cannone, J. J / 2002 / The Comparative RNA Web (CRW) Site: an online database of comparative sequence and structure information for ribosomal, intron, and other RNAs / BMC Bi
Capel, M. S / 1987 / A complete mapping of the proteins in the small ribosomal subunit of Escherichia coli. / Science 238 : 1403 ~ 1406
Bakin, A / 1994 / The single pseudouridine residue in Escherichia coli 16S RNA is located at position 516 / Nucleic Acids Res. 22 : 3681 ~ 3684
Bock, A / 1979 / Ribosomal ambiguity (ram) mutations facilitate diyhydrostreptomycin binding to ribosomes / FEBS Lett. 104 : 317 ~ 321
Cannone, J. J / 2002 / The Comparative RNA Web (CRW) Site: an online database of comparative sequence and structure information for ribosomal, intron, and other RNAs / BMC Bi
Capel, M. S / 1987 / A complete mapping of the proteins in the small ribosomal subunit of Escherichia coli. / Science 238 : 1403 ~ 1406
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