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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제1호
- 발행연도
- 2011.1
- 수록면
- 20 - 27 (8page)
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초록· 키워드
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The purification and characterization of a Mn²+-dependent alkaline serine protease produced by Bacillus pumilus TMS55 were investigated. The enzyme was purified in three steps: concentrating the crude enzyme using ammonium sulfate precipitation, followed by gel filtration and cation-exchange chromatography. The purified protease had a molecular mass of approximately 35 kDa,was highly active over a broad pH range of 7.0 to 12.0,and remained stable over a pH range of 7.5 to 11.5. The optimum temperature for the enzyme activity was found to be 60oC. PMSF and AEBSF (1 mM) significantly inhibited the protease activity, indicating that the protease is a serine protease. Mn²+ ions enhanced the activity and stability of the enzyme. In addition, the purified protease remained stable with oxidants (H₂O₂, 2%) and organic solvents (25%), such as benzene, hexane, and toluene.
Therefore, these characteristics of the protease and its dehairing ability indicate its potential for a wide range of commercial applications.
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참고문헌 (25)
참고문헌 신청
Akel, H / 2009 / Characterization of a purified thermostable protease from hyperthermophilic Bacillus strain HUTBS71 / Eur. J. Sci. Res 31 : 280 ~ 288
Alexander, K. T. W / 1988 / Enzymes in the tannery ? catalysts for progress / J. Am. Leather Chem. Assoc 83 : 287 ~ 316
Annapurna, R. A / 1996 / Eco-friendly enzymatic dehairing using extracellular protease from Bacillus species isolate / J. Am. Leather Chem. Assoc 91 : 115 ~ 119
Anwar, A / 2000 / Alkaline protease from Spilosoma obliqua: Potential applications in bio-formulations / Biotechnol. Appl. Biochem 31 : 85 ~ 89
Chandrasekaran,M / 1997 / Industrial enzymes from marine microorganisms: The Indian scenario / J. Mar. Biotechnol 5 : 86 ~ 89
Alexander, K. T. W / 1988 / Enzymes in the tannery ? catalysts for progress / J. Am. Leather Chem. Assoc 83 : 287 ~ 316
Annapurna, R. A / 1996 / Eco-friendly enzymatic dehairing using extracellular protease from Bacillus species isolate / J. Am. Leather Chem. Assoc 91 : 115 ~ 119
Anwar, A / 2000 / Alkaline protease from Spilosoma obliqua: Potential applications in bio-formulations / Biotechnol. Appl. Biochem 31 : 85 ~ 89
Chandrasekaran,M / 1997 / Industrial enzymes from marine microorganisms: The Indian scenario / J. Mar. Biotechnol 5 : 86 ~ 89
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