지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제10호
- 발행연도
- 2012.1
- 수록면
- 1,446 - 1,451 (6page)
이용수
초록· 키워드
오류제보하기
Levodopa or L-3,4-dihydroxyphenylalanine (L-DOPA) is the precursor of the neurotransmitter dopamine. L-DOPA is a famous treatment for Parkinson’s disease symptoms.
In this study, electroenzymatic synthesis of L-DOPA was performed in a three-electrode cell, comprising a Ag/AgCl reference electrode, a platinum wire auxiliary electrode,and a glassy carbon working electrode. L-DOPA had an oxidation peak at 376 mV and a reduction peak at -550 mV. The optimum conditions of pH, temperature,and amount of free tyrosinase enzyme were pH 7, 30oC,and 250 IU, respectively. The kinetic constant of the free tyrosinase enzyme was found for both cresolase and catacholase activity to be 0.25 and 0.4 mM, respectively. A cyclic voltammogram was used to investigate the electron transfer rate constant. The mean heterogeneous electron transfer rate (ke) was 5.8 × 10-4 cm/s. The results suggest that the electroenzymatic method could be an alternative way to produce L-DOPA without the use of a reducing agent such as ascorbic acid.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Andriani, D. / 2012 / Immobilization of cellulose from newly isolated strain Bacillus subtilis TD6 using calcium alginate as support material / Bioprocess Biosyst. Eng 35 : 2
Ardakani, M. M. / 2009 / Electrocatalytic oxidation of cysteine by indigo carmine modified glassy carbon electrode / J. Argent. Chem. Soc 97 : 106 ~ 115
Ates, S. / 2007 / Production of L-DOPA using Cu-alginate gel immobilizedtyrosinase in a batch and packed bed reactor / Enzyme Microb.Technol 40 : 683 ~ 687
Barbero, C. / 1988 / Studies of surfacemodified glassy carbon electrode obtained by electrochemical treatment / J. Electroanal. Chem 248 : 321 ~ 340
Bard, A. J. / 2001 / Electrochemical Methods: Fundamentals and Applications, 2nd Ed / Wiley : 92 ~ 98
Ardakani, M. M. / 2009 / Electrocatalytic oxidation of cysteine by indigo carmine modified glassy carbon electrode / J. Argent. Chem. Soc 97 : 106 ~ 115
Ates, S. / 2007 / Production of L-DOPA using Cu-alginate gel immobilizedtyrosinase in a batch and packed bed reactor / Enzyme Microb.Technol 40 : 683 ~ 687
Barbero, C. / 1988 / Studies of surfacemodified glassy carbon electrode obtained by electrochemical treatment / J. Electroanal. Chem 248 : 321 ~ 340
Bard, A. J. / 2001 / Electrochemical Methods: Fundamentals and Applications, 2nd Ed / Wiley : 92 ~ 98
이 논문의 저자 정보
최근 본 자료
댓글(0)
0