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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제5호
- 발행연도
- 2013.1
- 수록면
- 661 - 667 (7page)
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Lipase-producing bacterial strains were isolated from Antarctic soil samples using the tricaprylin agar plate method. Seven strains with relatively strong lipase activities were selected. All of them turned out to be Bacillus pumilus strains by the 16S rRNA gene sequence analysis. Their corresponding lipase genes were cloned,sequenced, and compared. Finally, three different Bacillus pumilus lipases (BPL1, BPL2, and BPL3) were chosen.
Their amino acid sequence identities were in the range of 92-98% with the previous Bacillus pumilus lipases. Their optimum temperatures and pHs were measured to be 40oC and pH 9. Lipase BPL1 and lipase BPL2 were stable up to 30oC, whereas lipase BPL3 was stable up to 20oC.
Lipase BPL2 was stable within a pH range of 6-10,whereas lipase BPL1 and lipase BPL3 were stable within a pH range of 5-11, showing strong alkaline tolerance. All these lipases exhibited high hydrolytic activity toward pnitrophenyl caprylate (C8). In addition, lipase BPL1showed high hydrolytic activity toward tributyrin, whereas lipase BPL2 and lipase BPL3 hydrolyzed tricaprylin and castor oil preferentially. These results demonstrated that the three Antarctic Bacillus lipases were alkaliphilic and had a substrate preference toward short- and mediumchain triglycerides. These Antarctic Bacillus lipases might be used in detergent and food industries.
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참고문헌 (24)
참고문헌 신청
Akbulut, N. / 2013 / Improved activity and thermostability of Bacillus pumilus lipase by directed evolution / J. Biotechnol : 016 ~
Alquati, C. / 2002 / The cold-active lipase of Pseudomonas fragi. Heterologous expression, biochemical characterization and molecular modeling / Eur. J. Biochem 269 : 3321 ~
Arpigny, J. L. / 1993 / Cloning, sequence and structural features of a lipase from the antarctic facultative psychrophile Psychrobacter immobilis B10 / Biochim. Biophys. Acta
Bell, P. J. / 2002 / Prospecting for novel lipase genes using PCR / Microbiology 148 : 2283 ~ 2291
Bustos-Jaimes, I. / 2010 / Kinetic studies of Gly28:Ser mutant form of Bacillus pumilus lipase: Changes in k(cat) and thermal dependence / Biochim. Biophys. Acta 1804 : 2222
Alquati, C. / 2002 / The cold-active lipase of Pseudomonas fragi. Heterologous expression, biochemical characterization and molecular modeling / Eur. J. Biochem 269 : 3321 ~
Arpigny, J. L. / 1993 / Cloning, sequence and structural features of a lipase from the antarctic facultative psychrophile Psychrobacter immobilis B10 / Biochim. Biophys. Acta
Bell, P. J. / 2002 / Prospecting for novel lipase genes using PCR / Microbiology 148 : 2283 ~ 2291
Bustos-Jaimes, I. / 2010 / Kinetic studies of Gly28:Ser mutant form of Bacillus pumilus lipase: Changes in k(cat) and thermal dependence / Biochim. Biophys. Acta 1804 : 2222
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