지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제8호
- 발행연도
- 2014.1
- 수록면
- 1,044 - 1,050 (7page)
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Since there is no consensus about the most reliable assays to detect invasive aspergillosis from samples obtained by minimally invasive or noninvasive methods, we compared the efficacy of an enzyme-linked immunosorbent assay (ELISA) for galactomannan (GM) detection and quantitative real-time PCR assay (qRT-PCR) for the diagnosis of invasive pulmonary aspergillosis. Neutropenic, male Sprague-Dawley rats (specific pathogen free; 8 weeks old; weight, 200 ± 20 g) were immunosuppressed with cyclophosphamide and infected with Aspergillus fumigatus intratracheally. Tissue and whole blood samples were harvested on days 1, 3, 5, and 7 post-infection and examined with GM ELISA and qRT-PCR. The A. fumigatus DNA detection sequence was detected in the following number of samples from 12 immunosuppressed, infected rats examined on the scheduled days: day 1 (0/12), day 3 (0/12), day 5 (6/12), and day 7 (8/12) post-infection. The sensitivity and specificity of the qRT-PCR assay was 29.2% and 100%, respectively. Receiver operating characteristic curve (ROC) analysis indicated a Ct (cycle threshold) cut-off value of 15.35, and the area under the curve (AUC) was 0.627. The GM assay detected antigen in sera obtained on day 1 (5/12), day 3 (9/ 12), day 5 (12/12), and day 7 (12/12) post-infection, and thus had a sensitivity of 79.2% and a specificity of 100%. The ROC of the GM assay indicated that the optimal Ct cut-off value was 1.40 (AUC, 0.919). The GM assay was more sensitive than the qRT-PCR assay in diagnosing invasive pulmonary aspergillosis in rats.
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참고문헌 (19)
참고문헌 신청
Aquino VR / 2012 / The performance of real-time PCR, galactomannan, and fungal culture in the diagnosis of invasive aspergillosis in ventilated patients with chronic obstruct
Becker MJ / 2000 / Quantitative galactomannan detection is superior to PCR in diagnosing and monitoring invasive pulmonary aspergillosis in an experimental rat model / J. Cli
Chamilos G / 2006 / Invasive fungal infections in patients with hematologic malignancies in a tertiary care cancer center : an autopsy study over a 15-year period(1989-2003)
Groll AH / 2009 / Current challenges in the diagnosis and management of invasive fungal infections:report from the 15th International Symposium on Infections in the Immunocom
Hadrich I / 2011 / Comparison of PCR-ELISA and real-time PCR for invasive aspergillosis diagnosis in patients with hematological malignancies / Med. Mycol 49 : 489 ~ 494
Becker MJ / 2000 / Quantitative galactomannan detection is superior to PCR in diagnosing and monitoring invasive pulmonary aspergillosis in an experimental rat model / J. Cli
Chamilos G / 2006 / Invasive fungal infections in patients with hematologic malignancies in a tertiary care cancer center : an autopsy study over a 15-year period(1989-2003)
Groll AH / 2009 / Current challenges in the diagnosis and management of invasive fungal infections:report from the 15th International Symposium on Infections in the Immunocom
Hadrich I / 2011 / Comparison of PCR-ELISA and real-time PCR for invasive aspergillosis diagnosis in patients with hematological malignancies / Med. Mycol 49 : 489 ~ 494
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