지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제12호
- 발행연도
- 2014.1
- 수록면
- 1,622 - 1,628 (7page)
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초록· 키워드
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DagA, a β-agarase, was produced by cultivating a recombinant Streptomyces lividans in a glucose medium or a mixed-sugar medium simulating microalgae hydrolysate. The optimum composition of the glucose medium was identified as 25 g/l glucose, 10 g/l yeast extract, and 5 g/l MgCl2·6H2O. With this, a DagA activity of 7.26 U/ml could be obtained. When a mixedsugar medium containing 25 g/l of sugars was used, a DagA activity of 4.81 U/ml was obtained with very low substrate utilization efficiency owing to the catabolic repression of glucose against the other sugars. When glucose and galactose were removed from the medium, an unexpectedly high DagA activity of about 8.7 U/ml was obtained, even though a smaller amount of sugars was used. It is recommended for better substrate utilization and process economics that glucose and galactose be eliminated from the medium, by being consumed by some other useful applications, before the production of DagA.
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참고문헌 (25)
참고문헌 신청
Araki C. / 1956 / Structure of the agarose constituent of agaragar / Bull. Chem. Soc. Jpn 29 : 543 ~ 544
B ibb MJ / 1987 / The agarase gene (dagA) of Streptomyces coelicolor A3(2): affinity purification and characterization of the cloned geneproduct / J. Gen. Microbiol 133 : 208
BANDI / 2005 / Statistical Approach to Development of Culture Medium for Ansamitocin P-3 Production with Actinosynnema pretiosum ATCC 31565 / Journal of Microbiology and Biot
Box GEP / 1951 / On the experimental attainment of optimum conditions / J. R. Stat. Soc. Series B Stat. Methodol 13 : 1 ~ 45
Brown MR. / 1991 / The amino-acid and sugar composition of 16 species of microalgae used in mariculture / J. Exper. Marine Biol. Ecol 145 : 79 ~ 99
B ibb MJ / 1987 / The agarase gene (dagA) of Streptomyces coelicolor A3(2): affinity purification and characterization of the cloned geneproduct / J. Gen. Microbiol 133 : 208
BANDI / 2005 / Statistical Approach to Development of Culture Medium for Ansamitocin P-3 Production with Actinosynnema pretiosum ATCC 31565 / Journal of Microbiology and Biot
Box GEP / 1951 / On the experimental attainment of optimum conditions / J. R. Stat. Soc. Series B Stat. Methodol 13 : 1 ~ 45
Brown MR. / 1991 / The amino-acid and sugar composition of 16 species of microalgae used in mariculture / J. Exper. Marine Biol. Ecol 145 : 79 ~ 99
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