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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제5호
- 발행연도
- 2016.1
- 수록면
- 946 - 952 (7page)
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초록· 키워드
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The eglS gene in Bacillus amyloliquefaciens encodes an endo-β-1,4-glucanase that belongs to glycosyl hydrolase family 5. In this study, a disruption mutant of gene eglS was constructed to examine its role in bacterial adaptation in plants. The mutant TB2k, eglS gene inactivated bacterial strain, was remarkably impaired in extracellular cellulase activity. When inoculated on Brassica campestris, the TB2k population was reduced by more than 60% compared with the wild-type strain in the root, stem, and leaf tissues. Overexpression of eglS in the wild-type strain increased the bacteria population in the plant tissues. Further studies revealed that the transcription level of eglS was correlated with bacterial population. These data demonstrate that endo-β-1,4-glucanase of B. amyloliquefaciens is required for its optimal endophytic colonization.
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참고문헌 (23)
참고문헌 신청
Balsanelli E / 2010 / Herbaspirillum seropedicae rfbB and rfbC genes are required for maize colonization / Environ. Microbiol 12:2233~2244
Cho KM / 2008 / Cloning of two cellulase genes from endophytic Paenibacillus polymyxa GS01 and comparison with cel 44Cman 26A / J. Basic Microb 48:464~472
Compant S / 2010 / Plant growthpromoting bacteria in the rhizo and endosphere of plants:their role, colonization, mechanisms involved and prospects for utilization / Soil Biol. Biochem 42:669~678
Compant S / 2005 / Endophytic colonization of Vitis vinifera L. by plant growth-promoting bacterium Burkholderia sp. strain PsJN / Appl. Environ. Microbiol 71:1685~1693
Fan XJ / 2008 / Cloning and expression of β-1,4-endoglucanase gene from endophytic Bacillus amyloliquefaciens TB2 / Chin. J. Trop. Crops 29:443~449
Cho KM / 2008 / Cloning of two cellulase genes from endophytic Paenibacillus polymyxa GS01 and comparison with cel 44Cman 26A / J. Basic Microb 48:464~472
Compant S / 2010 / Plant growthpromoting bacteria in the rhizo and endosphere of plants:their role, colonization, mechanisms involved and prospects for utilization / Soil Biol. Biochem 42:669~678
Compant S / 2005 / Endophytic colonization of Vitis vinifera L. by plant growth-promoting bacterium Burkholderia sp. strain PsJN / Appl. Environ. Microbiol 71:1685~1693
Fan XJ / 2008 / Cloning and expression of β-1,4-endoglucanase gene from endophytic Bacillus amyloliquefaciens TB2 / Chin. J. Trop. Crops 29:443~449
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