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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제5호
- 발행연도
- 2016.1
- 수록면
- 837 - 845 (9page)
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초록· 키워드
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A novel endodextranase isolated from Paenibacillus sp. was found to produce isomaltotetraose and small amounts of cycloisomaltooligosaccharides with a degree of polymerization of 7–14 from dextran. To determine the active site, the enzyme was modified with 1-ethyl-3-[3- (dimethylamino)-propyl]-carbodiimide (EDC) and α-epoxyalkyl α-glucosides (EAGs), an affinity labeling reagent. The inactivation followed pseudo first-order kinetics. Kinetic analysis and chemical modification using EDC and EAGs indicated that carboxyl groups are essential for the enzymatic activity. Three Asp and one Glu residues were identified as candidate catalytic amino acids, since these residues are completely conserved across the GH family of 66 enzymes. Replacement of Asp189, Asp340, or Glu412 completely abolished the enzyme activity, indicating that these residues are essential for catalytic activity.
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참고문헌 (30)
참고문헌 신청
Aoki H / 1997 / A classification of dextran-hydrolysing enzymes based on amino-acid-sequence similarities / Biochem. J 323:859~861
Barrett JF / 1987 / Purification and partial characterization of the multicomponent dextranase complex of Streptococcus sorbrinus and cloning of the dextranase gene / Infect. Immun 55:792~802
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72:248~254
Datta AK / 1995 / Efficient amplification using ‘megaprimer’ by asymmetric polymerase chain reaction / Nucleic Acids Res 23:4530~4531
Dixon M / 1979 / Enzymes / Academic Press :138~164
Barrett JF / 1987 / Purification and partial characterization of the multicomponent dextranase complex of Streptococcus sorbrinus and cloning of the dextranase gene / Infect. Immun 55:792~802
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72:248~254
Datta AK / 1995 / Efficient amplification using ‘megaprimer’ by asymmetric polymerase chain reaction / Nucleic Acids Res 23:4530~4531
Dixon M / 1979 / Enzymes / Academic Press :138~164
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