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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제8호
- 발행연도
- 2012.1
- 수록면
- 1,165 - 1,169 (5page)
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초록· 키워드
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In April 2009, the H1N1 pandemic influenza virus emerged as a novel influenza virus. The aim of this study was to compare the performances of several molecular assays, including conventional reverse transcription polymerase chain reaction (RT-PCR), two real-time reverse transcription (rRT)-PCRs, and two multiplex RTPCRs.
A total of 381 clinical specimens were collected from patients (223 men and 158 women), and both the Seeplex RV7 assay and rRT-PCR were ordered on different specimens within one week after collection. The concordance rate for the two methods was 87% (332/381),and the discrepancy rate was 13% (49/381). The positive rates for the molecular assays studied included 93.1% for the multiplex Seeplex RV7 assay, 93.1% for conventional reverse transcription (cRT)-PCR, 89.7% for the multiplex Seeplex Flu ACE Subtyping assay, 82.8% for protocol B rRT-PCR, and 58.6% for protocol A rRT-PCR. Our results showed that the multiplex Seeplex assays and the cRT-PCR yielded higher detection rates than rRT-PCRs for detecting the influenza A (H1N1) virus. Although the multiplex Seeplex assays had the advantage of simultaneous detection of several viruses, they were time-consuming and troublesome. Our results show that, although rRTPCR had the advantage, the detection rates of the molecular assays varied depending upon the source of the influenza A (H1N1)v virus. Our findings also suggest that rRT-PCR sometimes detected virus in extremely low abundance and thus required validation of analytical performance and clinical correlation.
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참고문헌 (20)
참고문헌 신청
Bellau-Pujol, S. / 2005 / Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses / J. Virol. Methods 126 : 53 ~ 63
Carr, M. J. / 2009 / Development of a real-time RT-PCR for the detection of swine-lineage influenza A (H1N1) virus infections / J. Clin. Virol 45 : 196 ~ 199
Hall, R. J. / 2009 / Rapid method to support diagnosis of swine origin influenza virus infection by sequencing of real-time PCR amplicons from diagnostic assays / J. Clin. Mi
Hindiyeh, M. / 2005 / Evaluation of a multiplex real-time reverse transcriptase PCR assay for detection and differentiation of influenza viruses A and B during the 2001-2002
Hymas, W. C. / 2010 / Development of a multiplex real-time RT-PCR assay for detection of influenza A, influenza B, RSV and typing of the 2009-H1N1 influenza virus / J. Virol.
Carr, M. J. / 2009 / Development of a real-time RT-PCR for the detection of swine-lineage influenza A (H1N1) virus infections / J. Clin. Virol 45 : 196 ~ 199
Hall, R. J. / 2009 / Rapid method to support diagnosis of swine origin influenza virus infection by sequencing of real-time PCR amplicons from diagnostic assays / J. Clin. Mi
Hindiyeh, M. / 2005 / Evaluation of a multiplex real-time reverse transcriptase PCR assay for detection and differentiation of influenza viruses A and B during the 2001-2002
Hymas, W. C. / 2010 / Development of a multiplex real-time RT-PCR assay for detection of influenza A, influenza B, RSV and typing of the 2009-H1N1 influenza virus / J. Virol.
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