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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제12호
- 발행연도
- 2010.1
- 수록면
- 1,664 - 1,671 (8page)
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초록· 키워드
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A new biosurfactant producer was isolated from palm-oilcontaminated soil and later identified through morphology and DNA sequencing as the yeast-like fungus Exophiala dermatitidis. Biosurfactant production was catalyzed by vegetable oil, supplemented with a basal medium. The culture conditions that provided the biosurfactant with the highest surface activity were found to be 5% palm oil with 0.08% NH4NO3, at a pH of 5.3, with shaking at 200 rpm, and a temperature of 30oC for a 14-day period of incubation. The biosurfactant was purified, in accordance with surfactant properties, by solvent fractionation using silica gel column chromatography. The chemical structure of the strongest surface-active compound was elucidated through the use of NMR and mass spectroscopy, and noted to be monoolein, which then went on to demonstrate antiproliferative activity against cervical cancer (HeLa)and leukemia (U937) cell lines in a dose-dependent manner. Interestingly, no cytotoxicity was observed with normal cells even when high concentrations were used.
Cell and DNA morphological changes, in both cancer cell lines, were observed to be cell shrinkage, membrane blebbling, and DNA fragmentation.
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참고문헌 (23)
참고문헌 신청
Altschul, S. F / 1997 / Gapped BLAST and PSIBLAST: A new generation of protein database search programs / Nucleic Acids Res 25 : 3389 ~ 3402
Banat, I. M / 2010 / Microbial biosurfactants production, applications and future potential / Appl. Microbiol. Biotechnol 87 : 427 ~ 444
Bergsson, G / 2002 / Bactericidal effects of fatty acids and monoglycerides on Helicobacter pylori / Int. J. Antimicrob. Agents 20 : 258 ~ 262
Bodour, A. A / 1998 / Application of modified drop-collapse technique for surfactant quantitation and screening of biosurfactant-producing microorganisms / J. Microbiol. Meth
Cameotra, S. S / 2009 / Synthesis of rhamnolipid biosurfactant and mode of hexadecane uptake by Pseudomonas species / Microb. Cell Fact 8 : 16 ~ 22
Banat, I. M / 2010 / Microbial biosurfactants production, applications and future potential / Appl. Microbiol. Biotechnol 87 : 427 ~ 444
Bergsson, G / 2002 / Bactericidal effects of fatty acids and monoglycerides on Helicobacter pylori / Int. J. Antimicrob. Agents 20 : 258 ~ 262
Bodour, A. A / 1998 / Application of modified drop-collapse technique for surfactant quantitation and screening of biosurfactant-producing microorganisms / J. Microbiol. Meth
Cameotra, S. S / 2009 / Synthesis of rhamnolipid biosurfactant and mode of hexadecane uptake by Pseudomonas species / Microb. Cell Fact 8 : 16 ~ 22
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