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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제11호
- 발행연도
- 2012.1
- 수록면
- 1,540 - 1,548 (9page)
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초록· 키워드
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Manganese peroxidase (MnP) was isolated from the culture filtrate of the wood log mushroom Stereum ostrea (S. ostrea), grown on Koroljova medium, and then purified by ammonium sulfate [70% (w/v)] fractionation, DEAEcellulose anion exchange chromatography, and Sephadex G-100 column chromatography, with an attainment of 88.6-fold purification and the recovery of 22.8% of initial activity. According to SDS-PAGE the molecular mass of the MnP was 40 kDa. The optimal pH and temperature were found to be 4.5 and 35oC, respectively. The enzyme was stable even after exposure to a pH range of 4.5 to 6.0,and at temperatures of up to 35oC at a pH of 4.5 for 1h.
The Km and Vmax values for the substrate phenol red were found to be 8 μm and 111.14 U/mg of protein, respectively.
The MnP also oxidized other substrates such as guaiacol,DMP, and veratryl alcohol. Sodium azide, EDTA, SDS,Cu2+, and Fe2+, at 1-5 mM, strongly inhibited enzyme activity, whereas Ca2+ and Zn2+ increased enzyme activity.
The participation of the purified enzyme in the decolorization of dyes suggests that S. ostrea manganese peroxidase could be effectively employed in textile industries.
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참고문헌 (21)
참고문헌 신청
Arora, D. S. / 2005 / Production of ligninolytic enzymes by Phlebia floridensis / World J. Microbiol. Biotechnol 21 : 1021 ~ 1028
Baborova, P. / 2006 / Purification of a new manganese peroxidase of the white-rot fungus Irpex lacteus and degradation of polycyclic aromatic hydrocarbons by the enzyme / Res
Bonnarme, P. / 1990 / Regulation of lignin peroxidases and manganese-dependent peroxidases from lignindegrading white-rot fungi / Appl. Environ. Microbiol 56 : 210 ~ 217
Cheng, X. / 2007 / Purification of a new manganese peroxidase of the white-rot fungus Schizophyllum sp. F17, and decolorization of azo dyes by the enzyme / Enzyme Microb. Tec
Christian, C. C. / 2003 / Decolorization of sulfonaphthalein dyes by manganese peroxidase activity of the white-rot fungus Phanerochaete chrysosporium / Folia Microbiol 6 : 7
Baborova, P. / 2006 / Purification of a new manganese peroxidase of the white-rot fungus Irpex lacteus and degradation of polycyclic aromatic hydrocarbons by the enzyme / Res
Bonnarme, P. / 1990 / Regulation of lignin peroxidases and manganese-dependent peroxidases from lignindegrading white-rot fungi / Appl. Environ. Microbiol 56 : 210 ~ 217
Cheng, X. / 2007 / Purification of a new manganese peroxidase of the white-rot fungus Schizophyllum sp. F17, and decolorization of azo dyes by the enzyme / Enzyme Microb. Tec
Christian, C. C. / 2003 / Decolorization of sulfonaphthalein dyes by manganese peroxidase activity of the white-rot fungus Phanerochaete chrysosporium / Folia Microbiol 6 : 7
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