지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제11호
- 발행연도
- 2013.1
- 수록면
- 1,598 - 1,609 (12page)
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초록· 키워드
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Organochlorine pesticide residues continue to remain as a major environmental threat worldwide. Lindane is an organochlorine pesticide widely used as an acaricide in medicine and agriculture. In the present study, a new lindane-degrading yeast strain, Pseudozyma VITJzN01, was identified as a copious producer of glycolipid biosurfactant. The glycolipid structure and type were elucidated by FTIR, NMR spectroscopy, and GC-MS analysis. The surface activity and stability of the glycolipid was analyzed. The glycolipids, characterized as mannosylerythritol lipids (MELs), exhibited excellent surface active properties and the surface tension of water was reduced to 29 mN/m. The glycolipid was stable over a wide range of pH,temperature, and salinity, showing a very low CMC of 25 mg/l. Bio-microemulsion of olive oil-in-water (O/W) was prepared using the purified biosurfactant without addition of any synthetic cosurfactants, for lindane solubilization and enhanced degradation assay in liquid and soil slurry. The O/W bio-microemulsions enhanced the solubility of lindane up to 40-folds. Degradation of lindane (700 mg/l) by VITJzN01 in liquid medium amended with bio-microemulsions was found to be enhanced by 36% in 2 days, compared with degradation in 12 days in the absence of bio-microemulsions. Lindane-spiked soil slurry incubated with bio-microemulsions also showed 20-40% enhanced degradation compared with the treatment with glycolipids or yeast alone. This is the first report on lindane degradation by Pseudozyma sp., and application of bio-microemulsions for enhanced lindane degradation. MEL-stabilized bio-microemulsions can serve as a potential tool for enhanced remediation of diverse lindanecontaminated environments.
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참고문헌 (22)
참고문헌 신청
Bodour AA / 2004 / Structure and characterization of flavolipids, a novel class of biosurfactants produced by Flavobacterium sp. strain MTN11 / Appl. Environ. Microbiol 70 :
Chakrabarty MM / 1969 / A simple photometric method for microdetermination of fatty acids in lipids / J. Am. Oil Chem. Soc 46 : 473 ~ 475
Chandankere R / 2013 / An efficient biosurfactant-producing and crude-oil emulsifying bacterium Bacillus methylotrophicus USTBa isolated from petroleum reservoir / Biochem. E
Cheng HR / 2006 / Extremely rapid extraction of DNA from bacteria and yeasts / Biotechnol. Lett 28 : 55 ~ 59
Dubois M / 1956 / Colorimetric method for determination of sugars and related substances / Anal. Chem 28 : 350 ~ 356
Chakrabarty MM / 1969 / A simple photometric method for microdetermination of fatty acids in lipids / J. Am. Oil Chem. Soc 46 : 473 ~ 475
Chandankere R / 2013 / An efficient biosurfactant-producing and crude-oil emulsifying bacterium Bacillus methylotrophicus USTBa isolated from petroleum reservoir / Biochem. E
Cheng HR / 2006 / Extremely rapid extraction of DNA from bacteria and yeasts / Biotechnol. Lett 28 : 55 ~ 59
Dubois M / 1956 / Colorimetric method for determination of sugars and related substances / Anal. Chem 28 : 350 ~ 356
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