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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제11호
- 발행연도
- 2015.1
- 수록면
- 1,835 - 1,841 (7page)
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A cell surface display system for heterologous expression of the multifunctional cellulase, CelEx-BR12, in Escherichia coli was developed using truncated E. coli outer membrane protein C (OmpC) as an anchor motif. Cell surface expression of CelEx-BR12 cellulase in E. coli harboring OmpC-fused CelEx-BR12, designated MC4100 (pTOCBR12), was confirmed by fluorescence-activated cell sorting and analysis of outer membrane fractions by western blotting, which verified the expected molecular mass of OmpC-fused CelEx-BR12 (~72 kDa). Functional evidence for exocellulase activity was provided by enzymatic assays of whole cells and outer membrane protein fractions from E. coli MC4100 (pTOCBR12). The stability of E. coli MC4100 (pTOCBR12) cellulase activity was tested by carrying out repeated reaction cycles, which demonstrated the reusability of recombinant cells. Finally, we showed that recombinant E. coli cells displaying the CelEx-BR12 enzyme on the cell surface were capable of growth using carboxymethyl cellulose as the sole carbon source.
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참고문헌 (24)
참고문헌 신청
Beguin P / 1994 / The biological degradation of cellulose / FEMS Microbiol. Rev 13 : 25 ~ 58
CHOI, JONG HYUN / 2005 / Display of Proteins on the Surface of Escherichia coli by C-Terminal Deletion Fusion to the Salmonella typhimurium OmpC / Journal of Microbiology and
Clarke AJ / 1997 / Biodegradation of Cellulose: Enzymology and Biotechnology / Technomic Publishing : 23 ~ 68
Cornelis P. / 2000 / Expressing genes in different Escherichia coli compartments / Curr. Opin. Biotechnol 11 : 450 ~ 454
He nrissat B / 1985 / Synergism of cellulases from Trichoderma reesei in the degradation of cellulose / Biotechnology 3 : 722 ~ 726
CHOI, JONG HYUN / 2005 / Display of Proteins on the Surface of Escherichia coli by C-Terminal Deletion Fusion to the Salmonella typhimurium OmpC / Journal of Microbiology and
Clarke AJ / 1997 / Biodegradation of Cellulose: Enzymology and Biotechnology / Technomic Publishing : 23 ~ 68
Cornelis P. / 2000 / Expressing genes in different Escherichia coli compartments / Curr. Opin. Biotechnol 11 : 450 ~ 454
He nrissat B / 1985 / Synergism of cellulases from Trichoderma reesei in the degradation of cellulose / Biotechnology 3 : 722 ~ 726
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