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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제3호
- 발행연도
- 2016.1
- 수록면
- 521 - 529 (9page)
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Extensive use of antibiotics over recent decades has led to bacterial resistance against antibiotics, including gentamicin, one of the most effective aminoglycosides. The emergence of resistance is problematic for hospitals, since gentamicin is an important broad-spectrum antibiotic for the control of bacterial pathogens in the clinic. Previous study to identify gentamicin resistance genes from environmental samples have been conducted using culturedependent screening methods. To overcome these limitations, we employed a metagenomebased culture-independent protocol to identify gentamicin resistance genes. Through functional screening of metagenome libraries derived from soil samples, a fosmid clone was selected as it conferred strong gentamicin resistance. To identify a specific functioning gene conferring gentamicin resistance from a selected fosmid clone (35–40 kb), a shot-gun library was constructed and four shot-gun clones (2–3 kb) were selected. Further characterization of these clones revealed that they contained sequences similar to that of the RNA ligase, T4 rnlA that is known as a toxin gene. The overexpression of the rnlA-like gene in Escherichia coli increased gentamicin resistance, indicating that this toxin gene modulates this trait. The results of our metagenome library analysis suggest that the rnlA-like gene may represent a new class of gentamicin resistance genes in pathogenic bacteria. In addition, we demonstrate that the soil metagenome can provide an important resource for the identification of antibiotic resistance genes, which are valuable molecular targets in efforts to overcome antibiotic resistance.
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참고문헌 (51)
참고문헌 신청
Allen HK / 2010 / Call of the wild: antibiotic resistance genes in natural environments / Nat. Rev. Microbiol 8:251~259
Allen HK / 2009 / Functional metagenomics reveals diverse betalactamases in a remote Alaskan soil / ISME J 3:243~251
Amann RI / 1995 / Phylogenetic identification and in situ detection of individual microbial cells without cultivation / Microbiol. Rev 59:143~169
Bateman A / 2004 / The Pfam protein families database / Nucleic Acids Res 32:D138~D141
Bland C / 2007 / CRISPR recognition tool (CRT): a tool for automatic detection of clustered regularly interspaced palindromic repeats / BMC Bioinformatics 8:209
Allen HK / 2009 / Functional metagenomics reveals diverse betalactamases in a remote Alaskan soil / ISME J 3:243~251
Amann RI / 1995 / Phylogenetic identification and in situ detection of individual microbial cells without cultivation / Microbiol. Rev 59:143~169
Bateman A / 2004 / The Pfam protein families database / Nucleic Acids Res 32:D138~D141
Bland C / 2007 / CRISPR recognition tool (CRT): a tool for automatic detection of clustered regularly interspaced palindromic repeats / BMC Bioinformatics 8:209
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