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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제11호
- 발행연도
- 2016.1
- 수록면
- 1,845 - 1,854 (10page)
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The transglycosylation activity of amylosucrase (ASase) has received significant attention owing to its use of an inexpensive donor, sucrose, and broad acceptor specificity, including glycone and aglycone compounds. The transglycosylation reaction of recombinant ASase from Deinococcus radiopugnans (DRpAS) was investigated using various phenolic compounds, and quercetin-3-O-rutinoside (rutin) was found to be the most suitable acceptor molecule used by DRpAS. Two amino acid residues in DRpAS variants (DRpAS Q299K and DRpAS Q299R), assumed to be involved in acceptor binding, were constructed by site-directed mutagenesis. Intriguingly, DRpAS Q299K and DRpAS Q299R produced 10-fold and 4-fold higher levels of rutin transglycosylation product than did the wild-type (WT) DRpAS, respectively. According to in silico molecular docking analysis, the lysine residue at position 299 in the mutants enables rutin to more easily position inside the active pocket of the mutant enzyme than in that of the WT, due to conformational changes in loop 4.
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참고문헌 (48)
참고문헌 신청
Arnold K / 2006 / The SWISSMODEL workspace: a Web-based environment for protein structure homology modelling / Bioinformatics 22:195~201
Arvanitoyannis IS / 2006 / Potential uses and applications of treated wine waste: a review / Int. J. Food Sci. Technol 41:475~487
Auffinger P / 2003 / The Mg2+ binding sites of t he 5 S rR NA l oop E m otif a s investigated b y molecular dynamics simulations / Chem. Biol 10:551~561
Azuma Y / 1986 / Pharmacological studies on the anti-inflammatory action of phenolic compounds / J. Dent. Res 65:53~56
Bae HK / 2002 / Modification of ascorbic acid using transglycosylation activity of Bacillus stearothermophilus maltogenic amylase to enhance its oxidative stability / J. Agric. Food Chem 50:3309~3316
Arvanitoyannis IS / 2006 / Potential uses and applications of treated wine waste: a review / Int. J. Food Sci. Technol 41:475~487
Auffinger P / 2003 / The Mg2+ binding sites of t he 5 S rR NA l oop E m otif a s investigated b y molecular dynamics simulations / Chem. Biol 10:551~561
Azuma Y / 1986 / Pharmacological studies on the anti-inflammatory action of phenolic compounds / J. Dent. Res 65:53~56
Bae HK / 2002 / Modification of ascorbic acid using transglycosylation activity of Bacillus stearothermophilus maltogenic amylase to enhance its oxidative stability / J. Agric. Food Chem 50:3309~3316
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