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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제11호
- 발행연도
- 2016.1
- 수록면
- 1,855 - 1,862 (8page)
이용수
초록· 키워드
오류제보하기
Cronobacter sakazakii (C. sakazakii) is a foodborne pathogen, posing a high risk of disease to infants and immunocompromised individuals. In order to develop a quick, easy, and sensitive assay for detecting C. sakazakii, a rabbit anti-C. sakazakii immunoglobulin G (IgG) was developed using sonicated cell protein from C. sakazakii. The developed anti-C. sakazakii (IgG) was of good quality and purity, as well as species-specific. The developed rabbit anti-C.
sakazakii IgG was attached to the surface of a sulforhodamine B-encapsulated liposome to form an immunoliposome. A test strip was then prepared by coating goat anti-rabbit IgG onto the control line and rabbit anti-C. sakazakii IgG onto the test line, respectively, of a plastic-backed nitrocellulose membrane. A purple color signal both on the test line and the control line indicated the presence of C. sakazakii in the sample, whereas purple color only on the control line indicated the absence of C. sakazakii in the sample. This immunochromatographic strip assay could produce results in 15 min with a limit of detection of 107 CFU/ml in C. sakazakii culture. The immunochromatographic strip assay also showed very good specificity without cross-reactivity with other tested Cronobacter species. Based on these results, the developed immunochromatographic strip assay is efficient for the detection of C. sakazakii and has high potential for on-site detection.
목차
등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Beuchat LR / 2009 / Cronobacter sakazakii in foods and factors affecting its survival, growth, and inactivation / Int. J. Food Microbiol 136 : 204 ~ 213
Blazkova M / 2011 / Immunochromatographic strip test for detection of genus Cronobacter / Biosens. Bioelectron 26 : 2828 ~ 2834
Bochot A / 2012 / Liposomes for intravitreal drug delivery: a state of the art / J. Control. Release 161 : 628 ~ 634
Bowen AB / 2006 / Invasive Enterobacter sakazakii disease in infants / Emerg. Infect. Dis 12 : 1185 ~ 1189
Chap J / 2009 / International survey of Cronobacter sakazakii and other Cronobacter spp. in follow up formulas and infant foods / Int. J. Food Microbiol 136 : 185 ~ 188
Blazkova M / 2011 / Immunochromatographic strip test for detection of genus Cronobacter / Biosens. Bioelectron 26 : 2828 ~ 2834
Bochot A / 2012 / Liposomes for intravitreal drug delivery: a state of the art / J. Control. Release 161 : 628 ~ 634
Bowen AB / 2006 / Invasive Enterobacter sakazakii disease in infants / Emerg. Infect. Dis 12 : 1185 ~ 1189
Chap J / 2009 / International survey of Cronobacter sakazakii and other Cronobacter spp. in follow up formulas and infant foods / Int. J. Food Microbiol 136 : 185 ~ 188
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