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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제2호
- 발행연도
- 2017.1
- 수록면
- 277 - 288 (12page)
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Rhizomucor miehei NRRL 5282 and Rhizopus oryzae NRRL 1526 can produce lipases with high synthetic activities in wheat bran-based solid-state culture. In this study, the purification and biochemical characterization of the lipolytic activities of these lipases are presented. SDSPAGE indicated a molecular mass of about 55 and 35 kDa for the purified R. miehei and Rh. oryzae enzymes, respectively. p-Nitrophenyl palmitate (pNPP) hydrolysis was maximal at 40°C and pH 7.0 for the R. miehei lipase, and at 30°C and pH 5.2 for the Rh. oryzae enzyme. The enzymes showed almost equal affinity to pNPP, but the Vmax of the Rh. oryzae lipase was about 1.13 times higher than that determined for R. miehei using the same substrate. For both enzymes, a dramatic loss of activity was observed in the presence of 5 mM Hg2+, Zn2+, or Mn2+, 10 mM N-bromosuccinimide or sodium dodecyl sulfate, and 5-10% (v/v) of hexanol or butanol. At the same time, they proved to be extraordinarily stable in the presence of nhexane, cyclohexane, n-heptane, and isooctane. Moreover, isopentanol up to 10% (v/v) and propionic acid in 1 mM concentrations increased the pNPP hydrolyzing activity of R. miehei lipase. Both enzymes had 1,3-regioselectivity, and efficiently hydrolyzed p-nitrophenyl (pNP) esters with C8-C16 acids, exhibiting maximum activity towards pNP-caprylate (R. miehei) and pNP-dodecanoate (Rh. oryzae). The purified lipases are promising candidates for various biotechnological applications.
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참고문헌 (24)
참고문헌 신청
Chattopadhyay M / 1999 / Production and purification of lipase by a mutant strain of Rhizopus arrhizus / Folia Microbiol 44 : 37 ~ 40
Ferreira JA / 2013 / Zygomycetes-based biorefinery: present status and future prospects / Bioresour. Technol 135 : 523 ~ 532
Haas MJ / 1992 / Purification and characterization of an extracellular lipase from the fungus Rhizopus delemar / Lipids 27 : 571 ~ 576
Hasan F / 2006 / Industrial applications of microbial lipases / Enzyme Microb. Technol 39 : 235 ~ 251
Hiol A / 2000 / Purification and characterization of an extracellular lipase f rom a t hermophilic Rhizopus oryzae strain isolated from palm fruit / Enzyme Microb. Technol 2
Ferreira JA / 2013 / Zygomycetes-based biorefinery: present status and future prospects / Bioresour. Technol 135 : 523 ~ 532
Haas MJ / 1992 / Purification and characterization of an extracellular lipase from the fungus Rhizopus delemar / Lipids 27 : 571 ~ 576
Hasan F / 2006 / Industrial applications of microbial lipases / Enzyme Microb. Technol 39 : 235 ~ 251
Hiol A / 2000 / Purification and characterization of an extracellular lipase f rom a t hermophilic Rhizopus oryzae strain isolated from palm fruit / Enzyme Microb. Technol 2
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