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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제2호
- 발행연도
- 2017.1
- 수록면
- 388 - 394 (7page)
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초록· 키워드
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Although several analytical methods for measuring total antioxidant capacity (TAC) have been applied to biological samples, there were often dissimilar results due to the different principles of methods applied. Thus, this study aimed to validate four conventional analytical methods for measuring plasma TAC, including the ABTS assay, DPPH assay, FRAP assay, and ORAC assay, by comparing with urinary 8-isoprostane concentration. In addition, TAC results were compared with antioxidant enzyme activities including superoxide dismutase (SOD) and glutathione peroxidase in erythrocyte, and catalase in plasma. Plasma TAC measure by ABTS assay was strongly correlated with the result by FRAP assay. Plasma TAC by FRAP and ORAC assays were negatively correlated with erythrocyte SOD activity. The agreement among the four TAC assay methods and 8-isoprostane was determined using 95% prediction limits of linear regression, expressed as the mean of 8-isoprostane ± 95% prediction limits. The ABTS method better agreed with 8-isoprostane than the other methods, demonstrating narrow prediction of limits. Furthermore, only plasma TAC determined by the ABTS assay was inversely correlated with urinary 8-isoprostane (r = -0.35, p < 0.05). In summary, the ABTS assay would be an appropriate method to measure overall plasma antioxidant capacity and predict the body’s antioxidant status.
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등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Altman DG / 1983 / Measurement in medicine: the analysis of method comparison studies / Statistician 32 : 307 ~ 317
Bland JM / 2003 / Applying the right statistics:analyses of measurement studies / Ultrasound Obstet. Gynecol 22 : 85 ~ 93
Font R / 2009 / Is there a relationship between cystatin C and inflammatory status, oxidative stress and other cardiovascular risk factors in non-diabetic patients with chron
Janaszewska A / 2002 / Assay of total antioxidant capacity: comparison of four methods as applied to human blood plasma / Scand. J. Clin. Lab. Invest 62 : 231 ~ 236
Jansen EH / 2013 / Comparative analysis of serum (anti)oxidative status parameters in healthy persons / Int. J. Mol. Sci 14 : 6106 ~ 6115
Bland JM / 2003 / Applying the right statistics:analyses of measurement studies / Ultrasound Obstet. Gynecol 22 : 85 ~ 93
Font R / 2009 / Is there a relationship between cystatin C and inflammatory status, oxidative stress and other cardiovascular risk factors in non-diabetic patients with chron
Janaszewska A / 2002 / Assay of total antioxidant capacity: comparison of four methods as applied to human blood plasma / Scand. J. Clin. Lab. Invest 62 : 231 ~ 236
Jansen EH / 2013 / Comparative analysis of serum (anti)oxidative status parameters in healthy persons / Int. J. Mol. Sci 14 : 6106 ~ 6115
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