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논문 기본 정보
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- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제17권 제2호
- 발행연도
- 2007.1
- 수록면
- 342 - 347 (6page)
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초록· 키워드
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Bacteriophage ΦFC1 integrase (MJ1) was previouslyshown to perform a site-specific recombination betwen aphage atachment site (attP) and a host attachment site (attB)in its host, Enterococus faecalis, and also in a non-hostbacterium, Escherichia coli. Here, we investigated biochemicalfeatures of MJ1 integrase. First, MJ1 integrase could performin vitro recombination between attP and attB in the absenceof additional factors. Second, MJ1 integrase interacted withatt sites. Electrophoretic mobility shift assays and DNase Ifootprinting revealed that MJ1 integrase could efficiently bindto all the att sites and that MJ1 integrase recognized relativelyshort sequences (~50 bp) containing an overlapping regionwithin attB and attP. These results demonstrate that MJ1integrase indeed catalyzes an integrative recombination betweenattP and , the mechanism of which might be simple andunidirectional, as found in serine integrases.
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참고문헌 (22)
참고문헌 신청
A / 1989 / Determinants of site-specific recombination in the lambdoid coliphage HK022 An evolutionary change in specificity : 695 ~ 717
Bertoni, C / 2006 / Enhancement of plasmidmediated gene therapy for muscular dystrophy by directed plasmid integration : 419 ~ 103 424
Bibb, L. A / 2002 / Integration and excision of the Mycobacterium tuberculosis prophage-like element 45 : 1515 ~ 1526
Bibb, L. A / 2005 / Integration and excision by the large serine recombinase ΦRv1 integrase 55 : 1896 ~ 1910
Breuner, A / 1999 / Novel organization of genes involved in prophage excision identified in the temperate lactococcal bacteriophage TP901-1 181 : 7291 ~ 7297
Bertoni, C / 2006 / Enhancement of plasmidmediated gene therapy for muscular dystrophy by directed plasmid integration : 419 ~ 103 424
Bibb, L. A / 2002 / Integration and excision of the Mycobacterium tuberculosis prophage-like element 45 : 1515 ~ 1526
Bibb, L. A / 2005 / Integration and excision by the large serine recombinase ΦRv1 integrase 55 : 1896 ~ 1910
Breuner, A / 1999 / Novel organization of genes involved in prophage excision identified in the temperate lactococcal bacteriophage TP901-1 181 : 7291 ~ 7297
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