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Antidiabetic effects of a novel microbial biopolymer(PGB)1 excreted from new Enterobacter sp. BL-2 weretested in the db/db mice. The animals were divided intonormal control, rosiglitazone (0.05%, wt/wt), low PGB1(0.1%, wt/wt), and high PGB1 (0.25%, wt/wt) groups. After 5weeks, the blood glucose levels of high PGB1 and rosiglitazonesuplemented groups were significantly lower than those ofthe control group. In hepatic glucose metabolic enzymeactivities, the glucokinase activities of PGB1 suplementedgroups were significantly higher than the control group,whereas the PEPCK activities were significantly lower. Thehigh PGB1 supplemented groups were significantly highercompared with the control group. Specifically, the insulin andglycogen increases were dose-responsive to PGB1 supplement.PGB1 supplement did not affect the IPGT and IPITT comparedwith the control group; however, rosiglitazone significantlyimproved IPIT. High PGB1 and rosiglitazone supplementationpreserved the appearance of islets and insulin-positive cells inimmunohistochemical photographs of the pancreas comparedwith the control group. These results demonstrated that highPGB1 (0.25% in the diet) supplementation seemingly contributesstimulating insulin secretion and enhancing the hepatic glucosemetabolic enzyme activities.

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