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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제7호
- 발행연도
- 2009.1
- 수록면
- 651 - 657 (7page)
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초록· 키워드
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Shifts in the activity and diversity of microbes involved
in aliphatic and aromatic hydrocarbon degradation in
contaminated soil were investigated. Subsurface soil was
collected from a gas station that had been abandoned
since 1995 owing to ground subsidence. The total petroleum
hydrocarbon content of the sample was approximately
2,100 mg/kg, and that of the soil below a gas pump was
over 23,000 mg/kg. Enrichment cultures were grown in
mineral medium that contained hexadecane (H) or
naphthalene (N) at a concentration of 200 mg/l. In the Henrichment
culture, a real-time PCR assay revealed that
the 16S rRNA gene copy number increased from 1.2×105
to 8.6×106 with no lag phase, representing an approximately
70-fold increase. In the N-enrichment culture, the 16S rRNA
copy number increased about 13-fold after 48 h, from
6.3×104 to 8.3×105. Microbial communities in the enrichment
cultures were studied by denaturing gradient gel electrophoresis
and by analysis of 16S rRNA gene libraries. Before the
addition of hydrocarbons, the gas station soil contained
primarily Alpha- and Gammaproteobacteria. During growth
in the H-enrichment culture, the contribution of Bacteriodetes
to the microbial community increased significantly. On
the other hand, during N-enrichment, the Betaproteobacteria
population increased conspicuously. These results suggest
that specific phylotypes of bacteria were associated with
the degradation of each hydrocarbon.
목차
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참고문헌 (22)
참고문헌 신청
BAEK, KYUNG-HWA / 2007 / Monitoring of Microbial Diversity and Activity During Bioremediation of Crude Oil-Contaminated Soil with Different Treatments / Journal of Microbiolo
Bordenave, S. / 2007 / Effects of heavy fuel oil on the bacterial community structure of a pristine microbial mat / Appl. Environ. Microbiol 73 : 6089 ~ 6097
Cheung, P. / 2001 / Mycobacterium diversity and pyrene mineralization in petroleum-contaminated soils / Appl. Environ. Microbiol 67 : 2222 ~ 2229
Dionisi, H. M. / 2004 / Abundance of dioxygenase genes similar to Ralstonia sp. strain U2 nagAc is correlated with naphthalene concentrations in coal tar-contaminated freshwa
Evans, F. F. / 2004 / Impact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms / FEMS Microbiol. Ecol 49 : 295 ~
Bordenave, S. / 2007 / Effects of heavy fuel oil on the bacterial community structure of a pristine microbial mat / Appl. Environ. Microbiol 73 : 6089 ~ 6097
Cheung, P. / 2001 / Mycobacterium diversity and pyrene mineralization in petroleum-contaminated soils / Appl. Environ. Microbiol 67 : 2222 ~ 2229
Dionisi, H. M. / 2004 / Abundance of dioxygenase genes similar to Ralstonia sp. strain U2 nagAc is correlated with naphthalene concentrations in coal tar-contaminated freshwa
Evans, F. F. / 2004 / Impact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms / FEMS Microbiol. Ecol 49 : 295 ~
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