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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제2호
- 발행연도
- 2012.1
- 수록면
- 166 - 169 (4page)
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In several biotechnological applications of living bacterial cells with inducible gene expression systems, the extent of overexpression and the specificity to the inducer are key elements. In the present study, we established the concentration ranges of Zn2+, Ni2+, Co2+, AsO2-, and Cd2+ ions that caused significant activation of the respective promoters of Synechocystis sp. without concomitant unspecific stress responses. The low expression levels can be increased up to 10-100-fold upon treatments with Cd2+, AsO2-, Zn2+,and Co2+ ions and up to 800-fold upon Ni2+ treatment.
These results facilitate the development of conditional gene expression systems in cyanobacteria.
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참고문헌 (10)
참고문헌 신청
Briggs, L. M. / 1990 / Copperinduced expression, cloning, and regulatory studies of the plastocyanin gene from the cyanobacterium Synechocystis sp. PCC-6803 / Plant Mol. Biol
Brostrom, C. O. / 1998 / Regulation of translational initiation during cellular responses to stress / Prog. Nucleic Acid Res. Mol. Biol 58 : 79 ~ 125
Cuypers, A. / 1999 / The chemical behaviour of heavy metals plays a prominent role in the induction of oxidative stress / Free Radic. Res 31 (Suppl) : S39 ~ S43
Huang, H. H. / 2010 / Design and characterization of molecular tools for a synthetic biology approach towards developing cyanobacterial biotechnology / Nucleic Acids Res 38 :
Kanesaki, Y. / 2007 / Histidine kinases play important roles in the perception and signal transduction of hydrogen peroxide in the cyanobacterium, Synechocystis sp. PCC 6803
Brostrom, C. O. / 1998 / Regulation of translational initiation during cellular responses to stress / Prog. Nucleic Acid Res. Mol. Biol 58 : 79 ~ 125
Cuypers, A. / 1999 / The chemical behaviour of heavy metals plays a prominent role in the induction of oxidative stress / Free Radic. Res 31 (Suppl) : S39 ~ S43
Huang, H. H. / 2010 / Design and characterization of molecular tools for a synthetic biology approach towards developing cyanobacterial biotechnology / Nucleic Acids Res 38 :
Kanesaki, Y. / 2007 / Histidine kinases play important roles in the perception and signal transduction of hydrogen peroxide in the cyanobacterium, Synechocystis sp. PCC 6803
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