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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제5호
- 발행연도
- 2012.1
- 수록면
- 668 - 673 (6page)
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초록· 키워드
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Biohydrogen production from organic wastewater by anaerobically activated sludge fermentation has already been extensively investigated, and it is known that hydrogen can be produced by glucose fermentation through three metabolic pathways, including the oxidative decarboxylation of pyruvic acid to acetyl-CoA, oxidation of NADH to NAD+, and acetogenesis by hydrogen-producing acetogens.
However, the exact or dominant pathways of hydrogen production in the anaerobically activated sludge fermentation process have not yet been identified. Thus, a continuous stirred-tank reactor (CSTR) was introduced and a specifically acclimated acidogenic fermentative microflora obtained under certain operation conditions. The hydrogen production activity and potential hydrogen-producing pathways in the acidogenic fermentative microflora were then investigated using batch cultures in Erlenmeyer flasks with a working volume of 500 ml. Based on an initial glucose concentration of 10 g/l, pH 6.0, and a biomass of 1.01 g/l of a mixed liquid volatile suspended solid (MLVSS), 247.7 ml of hydrogen was obtained after a 68 h cultivation period at 35 ± 1oC.
Further tests indicated that 69% of the hydrogen was produced from the oxidative decarboxylation of pyruvic acid, whereas the remaining 31% was from the oxidation of NADH to NAD+. There were no hydrogen-producing acetogens or they were unable to work effectively in the anaerobically activated sludge with a hydraulic retention time (HRT) of less than 8 h.
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참고문헌 (25)
참고문헌 신청
APHA. / 1995 / Standard Methods for The Examination of Water and Wastewater, 19th Ed / American Public Health Association
Adams, M. W. W. / 1990 / The structure and mechanism of ironhydrogenase / Biochim. Biophys. Acta 1020 : 115 ~ 145
Benemann, J. / 1996 / Hydrogen biotechnology: Progress and prospects / Nature Biotechnol 14 : 1101 ~ 1103
Bockris, J. M. / 2002 / The origin of ideas on a hydrogen economy and its solution to the decay of the environment / Int. J. Hydrogen Energy 27 : 731 ~ 740
Brosseau, J. D. / 1982 / Continuous microbial production of hydrogen gas / Int. J. Hydrogen Energy 7 : 623 ~ 628
Adams, M. W. W. / 1990 / The structure and mechanism of ironhydrogenase / Biochim. Biophys. Acta 1020 : 115 ~ 145
Benemann, J. / 1996 / Hydrogen biotechnology: Progress and prospects / Nature Biotechnol 14 : 1101 ~ 1103
Bockris, J. M. / 2002 / The origin of ideas on a hydrogen economy and its solution to the decay of the environment / Int. J. Hydrogen Energy 27 : 731 ~ 740
Brosseau, J. D. / 1982 / Continuous microbial production of hydrogen gas / Int. J. Hydrogen Energy 7 : 623 ~ 628
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