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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제4호
- 발행연도
- 2015.1
- 수록면
- 452 - 458 (7page)
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초록· 키워드
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The biocatalytic efficiency of recombinant Corynebacterium glutamicum ATCC 13032 expressing the secondary alcohol dehydrogenase of Micrococcus luteus NCTC2665 was studied. Recombinant C. glutamicum converts ricinoleic acid to a product, identified by gas chromatography/mass spectrometry as 12-ketooleic acid (12-oxo-cis-9-octadecenoic acid). The effects of pH, reaction temperature, and non-ionic detergent on recombinant C. glutamiucm whole cell bioconversion were examined. The determined optimal conditions for production of 12-ketooleic acid are pH 8.0, 35°C, and 0.05 g/l Tween80. Under these conditions, recombinant C. glutamicum produces 3.3 mM 12-ketooleic acid, with a 72% (mol/mol) maximum conversion yield, and 1.1 g/l/h volumetric productivity in 2 h; and 3.9 mM 12- ketooleic acid, with a 74% (mol/mol) maximum conversion yield, and 0.69 g/l/h maximum volumetric productivity in 4 h of fermentation. This study constitutes the first report of significant production of 12-ketooleic acid using a recombinant Corynebacterium glutamicumbased biocatalyst.
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참고문헌 (21)
참고문헌 신청
Doo EH / 2009 / Productivity of cyclohexanone oxidation of the recombinant Corynebacterium glutamicum expressing chnB of Acinetobacter calcoaceticus / J. Biotechnol 142 : 164
Hoffmann J / 2014 / Hyaluronic acid production with Corynebacterium glutamicum: effect of media composition on yield and molecular weight / J. Appl. Microbiol 117 : 663 ~ 678
Hou CT / 1991 / Production of a new compound, 7-10-dihydroxy-8-(E)-octadecenoic acid, by a Pseudomonas sp. PR3 / J. Ind. Microbiol 7 : 123 ~ 130
Hou CT / 1994 / Conversion of linoleic acid to 10-hydroxy-12(Z)-octadecenoic acid by Flavobacterium sp. (NRRL B-14859) / J. Am. Oil Chem. Soc 71 : 975 ~ 978
Hou CT / 1994 / Production of 10-ketostearic acid from oleic acid by Flavobacterium sp. s train DS5 (NRRL B-1485 9) / Appl. Environ. Microbiol 60 : 3760 ~ 3763
Hoffmann J / 2014 / Hyaluronic acid production with Corynebacterium glutamicum: effect of media composition on yield and molecular weight / J. Appl. Microbiol 117 : 663 ~ 678
Hou CT / 1991 / Production of a new compound, 7-10-dihydroxy-8-(E)-octadecenoic acid, by a Pseudomonas sp. PR3 / J. Ind. Microbiol 7 : 123 ~ 130
Hou CT / 1994 / Conversion of linoleic acid to 10-hydroxy-12(Z)-octadecenoic acid by Flavobacterium sp. (NRRL B-14859) / J. Am. Oil Chem. Soc 71 : 975 ~ 978
Hou CT / 1994 / Production of 10-ketostearic acid from oleic acid by Flavobacterium sp. s train DS5 (NRRL B-1485 9) / Appl. Environ. Microbiol 60 : 3760 ~ 3763
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