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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제6호
- 발행연도
- 2015.1
- 수록면
- 765 - 770 (6page)
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초록· 키워드
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Recent sequencing technology development has revolutionized fields of microbial ecology. MiSeq-based microbial community analysis allows us to sequence more than a few hundred samples at a time, which is far more cost-effective than pyrosequencing. The approach, however, has not been preferably used owing to computational difficulties of processing huge amounts of data as well as known Illumina-derived artefact problems with amplicon sequencing. The choice of assembly software to take advantage of paired-end sequencing and methods to remove Illumina artefacts sequences are discussed. The protocol we suggest not only removed erroneous reads, but also dramatically reduced computational workload, which allows even a typical desktop computer to process a huge amount of sequence data generated with Illumina sequencers. We also developed a Web interface (http://biotech.jejunu.ac.kr/ ~abl/16s/) that allows users to conduct fastq-merging and mothur batch creation. The study presented here should provide technical advantages and supports in applying MiSeq-based microbial community analysis.
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참고문헌 (21)
참고문헌 신청
Caporaso JG / 2010 / QIIME allows analysis of high-throughput community sequencing data / Nat. Methods 7 : 335 ~ 336
Caporaso JG / 2011 / Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample / Proc. Natl. Acad. Sci. USA 108 (Suppl 1) : 4516 ~ 4522
Caporaso JG / 2012 / Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms / ISME J 6 : 1621 ~ 1624
Cole JR / 2009 / The Ribosomal Database Project: improved alignments and new tools for rRNA analysis / Nucleic Acids Res 37 : D141 ~ D145
Degnan PH / 2012 / Illumina-based analysis of microbial community diversity / ISME J 6 : 183 ~ 194
Caporaso JG / 2011 / Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample / Proc. Natl. Acad. Sci. USA 108 (Suppl 1) : 4516 ~ 4522
Caporaso JG / 2012 / Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms / ISME J 6 : 1621 ~ 1624
Cole JR / 2009 / The Ribosomal Database Project: improved alignments and new tools for rRNA analysis / Nucleic Acids Res 37 : D141 ~ D145
Degnan PH / 2012 / Illumina-based analysis of microbial community diversity / ISME J 6 : 183 ~ 194
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